ArticleProceedings of the National Academy of Sciences of the United States of America2020
Connect-seq to superimpose molecular on anatomical neural circuit maps.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Mitochondrial calcium modulates odor-mediated behavioral plasticity in Caenorhabditis elegans.Molecules and cells · 2026Article
- Nos1 neurons in the paraventricular hypothalamic area modulate lipid metabolism via the sympathetic nervous system in male mice.Nature communications · 2026Article
- Deciphering molecular inputs to CRH neurons from POMC neurons.Fundamental research · 2026Article
- Olfactory inputs to appetite neurons in the hypothalamus.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Bisphenol A Impairs Spatial Memory by Inhibiting Hippocampal Excitatory Neuronal Response During Learning Events.Environment & health (Washington, D.C.) · 2025Article
- Single-cell genomics meets systems neuroscience: Insights from mapping the brain circuitry of stress.Journal of neuroendocrinology · 2025Review
- Molecular connectomics reveals a glucagon-like peptide 1-sensitive neural circuit for satiety.Nature metabolism · 2024Article
- In and out: Benchmarking in vitro, in vivo, ex vivo, and xenografting approaches for an integrative brain disease modeling pipeline.Stem cell reports · 2024Review
- Virus-Based Neural Circuit Tracing.Advances in neurobiology · 2024Review
- Unveiling Hypothalamic Molecular Signatures via Retrograde Viral Tracing and Single-Cell Transcriptomics.Scientific data · 2023Article
- Olfactory modulation of stress-response neural circuits.Experimental & molecular medicine · 2023Review
- Lateral hypothalamic leptin receptor neurons drive hunger-gated food-seeking and consummatory behaviours in male mice.Nature communications · 2023Article
- A method to investigate muscle target-specific transcriptional signatures of single motor neurons.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Recent advances in understanding neuronal diversity and neural circuit complexity across different brain regions using single-cell sequencing.Frontiers in neural circuits · 2023Review
- Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.Nature communications · 2022Article
- Ontogenetic rules for the molecular diversification of hypothalamic neurons.Nature reviews. Neuroscience · 2022Review
- Odor blocking of stress hormone responses.Scientific reports · 2022Article
- Advancements in the Quest to Map, Monitor, and Manipulate Neural Circuitry.Frontiers in neural circuits · 2022Review
- Shisa6 mediates cell-type specific regulation of depression in the nucleus accumbens.Molecular psychiatry · 2021Article
- Circadian neurons in the paraventricular nucleus entrain and sustain daily rhythms in glucocorticoids.Nature communications · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The mouse brain contains about 75 million neurons interconnected in a vast array of neural circuits. The identities and functions of individual neuronal components of most circuits are undefined. Here we describe a method, termed "Connect-seq," which combines retrograde viral tracing and single-cell transcriptomics to uncover the molecular identities of upstream neurons in a specific circuit and the signaling molecules they use to communicate. Connect-seq can generate a molecular map that can be superimposed on a neuroanatomical map to permit molecular and genetic interrogation of how the neuronal components of a circuit control its function. Application of this method to hypothalamic neurons controlling physiological responses to fear and stress reveals subsets of upstream neurons that express diverse constellations of signaling molecules and can be distinguished by their anatomical locations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.