ArticleCardiovascular research2021
Light sheet fluorescence microscopy as a new method for unbiased three-dimensional analysis of vascular injury.
Article in Cardiovascular research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
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- Development of Selective Plane Illumination Surface Enhanced Raman Scattering Spectral Microscopy for Improved Cellular Imaging.Journal of Raman spectroscopy : JRS · 2025Article
- Contrast-enhanced Micro-CT 3D visualization of cell distribution in hydrated human cornea.Heliyon · 2024Article
- Plexin D1 mediates disturbed flow-induced M1 macrophage polarization in atherosclerosis.Heliyon · 2023Article
- Detection of Choroidal Neovascularization Using Optical Tissue Transparency.Translational vision science & technology · 2023Article
- Three-dimensional cerebral vasculature topological parameter extraction of transgenic zebrafish embryos with a filling-enhancement deep learning network.Biomedical optics express · 2023Article
- Peptide Amphiphile Supramolecular Nanofibers Designed to Target Abdominal Aortic Aneurysms.ACS nano · 2022Article
- Multimodal Imaging of Target Detection Algorithm under Artificial Intelligence in the Diagnosis of Early Breast Cancer.Journal of healthcare engineering · 2022Article
- Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography.Scientific reports · 2021Article
- Light sheet fluorescence microscopy for quantitative three-dimensional imaging of vascular remodelling.Cardiovascular research · 2021Article
- Three-Dimensional Spheroids as In Vitro Preclinical Models for Cancer Research.Pharmaceutics · 2020Review
- X-ray Zernike phase contrast tomography: 3D ROI visualization of mm-sized mice organ tissues down to sub-cellular components.Biomedical optics express · 2020Article
- A Rat Carotid Artery Pressure-Controlled Segmental Balloon Injury with Periadventitial Therapeutic Application.Journal of visualized experiments : JoVE · 2020Article
- Development of Fractalkine-Targeted Nanofibers that Localize to Sites of Arterial Injury.Nanomaterials (Basel, Switzerland) · 2020Article
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6 authors.
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Abstract
aimsAssessment of preclinical models of vascular disease is paramount in the successful translation of novel treatments. The results of these models have traditionally relied on two-dimensional (2D) histological methodologies. Light sheet fluorescence microscopy (LSFM) is an imaging platform that allows for three-dimensional (3D) visualization of whole organs and tissues. In this study, we describe an improved methodological approach utilizing LSFM for imaging of preclinical vascular injury models while minimizing analysis bias. METHODS AND
resultsThe rat carotid artery segmental pressure-controlled balloon injury and mouse carotid artery ligation injury were performed. Arteries were harvested and processed for LSFM imaging and 3D analysis, as well as for 2D area histological analysis. Artery processing for LSFM imaging did not induce vessel shrinkage or expansion and was reversible by rehydrating the artery, allowing for subsequent sectioning and histological staining a posteriori. By generating a volumetric visualization along the length of the arteries, LSFM imaging provided different analysis modalities including volumetric, area, and radial parameters. Thus, LSFM-imaged arteries provided more precise measurements compared to classic histological analysis. Furthermore, LSFM provided additional information as compared to 2D analysis in demonstrating remodelling of the arterial media in regions of hyperplasia and periadventitial neovascularization around the ligated mouse artery.
conclusionLSFM provides a novel and robust 3D imaging platform for visualizing and quantifying arterial injury in preclinical models. When compared with classic histology, LSFM outperformed traditional methods in precision and quantitative capabilities. LSFM allows for more comprehensive quantitation as compared to traditional histological methodologies, while minimizing user bias associated with area analysis of alternating, 2D histological artery cross-sections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.