Evidence map›Paper›PMID 32060173›Full record

ArticleThe Plant cell2020

Metabolic Labeling of RNAs Uncovers Hidden Features and Dynamics of the Arabidopsis Transcriptome.

Emese Xochitl Szabo, Philipp Reichert, Marie-Kristin Lehniger, Marilena Ohmer, Marcella de Francisco Amorim, Udo Gowik, Christian Schmitz-Linneweber, Sascha Laubinger

Abstract read
In one paragraph

Article in The Plant cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  6. Molecular Analysis of miRNA Stability and Decay Dynamics by Northern Blot.Methods in molecular biology (Clifton, N.J.) · 2026
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  10. Review
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  13. RNA Decay Assay: 5-Ethynyl-Uridine Labeling and Chasing.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
  14. Article
  15. Article
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  18. Quantitative profiling of mNature communications · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Emese Xochitl SzaboInstitute for Biology and Environmental Science, University of Oldenburg, 26129 Oldenburg, Germany.ORCID 0000-0002-3603-0629
Philipp ReichertInstitute for Biology and Environmental Science, University of Oldenburg, 26129 Oldenburg, Germany.ORCID 0000-0002-5174-5446
Marie-Kristin LehnigerHumboldt-Universität Berlin, Institut für Biologie, 10115 Berlin, Germany.ORCID 0000-0002-5701-613X
Marilena OhmerCentre for Plant Molecular Biology, University of Tübingen, 72074 Tübingen, Germany.ORCID 0000-0002-1075-2084
Marcella de Francisco AmorimCentre for Plant Molecular Biology, University of Tübingen, 72074 Tübingen, Germany.ORCID 0000-0002-7915-3872
Udo GowikInstitute for Biology and Environmental Science, University of Oldenburg, 26129 Oldenburg, Germany.ORCID 0000-0002-8986-7318
Christian Schmitz-LinneweberHumboldt-Universität Berlin, Institut für Biologie, 10115 Berlin, Germany.ORCID 0000-0002-6125-4253
Sascha LaubingerInstitute for Biology and Environmental Science, University of Oldenburg, 26129 Oldenburg, Germany sascha.laubinger@uol.de.ORCID 0000-0002-6682-0728

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transcriptome analysis by RNA sequencing (RNA-seq) has become an indispensable research tool in modern plant biology. Virtually all RNA-seq studies provide a snapshot of the steady state transcriptome, which contains valuable information about RNA populations at a given time but lacks information about the dynamics of RNA synthesis and degradation. Only a few specialized sequencing techniques, such as global run-on sequencing, have been used to provide information about RNA synthesis rates in plants. Here, we demonstrate that RNA labeling with the modified, nontoxic uridine analog 5-ethynyl uridine (5-EU) in Arabidopsis (

Indexed as

Staining and LabelingArabidopsisHalf-LifeMicroRNAsRNA, AntisenseRNA, MessengerRNA, PlantRNA Processing, Post-TranscriptionalSeedlingsTranscriptomeUridineMicroRNAsRNA, AntisenseRNA, MessengerRNA, PlantUridine

Identifiers

PMID32060173
PMCPMC7145469

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.