ArticleInternational journal of molecular sciences2020
Identifying the Translatome of Mouse NEBD-Stage Oocytes via SSP-Profiling; A Novel Polysome Fractionation Method.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Regulation of Translation by PKA Signaling Pathway.International journal of molecular sciences · 2026Review
- Spatiotemporal dynamics and selectivity of mRNA translation during mouse pre-implantation development.Nucleic acids research · 2025Article
- Loss of ADAR1 protein induces changes in small RNA landscape in hepatocytes.RNA (New York, N.Y.) · 2024Article
- Article
- A genome-wide perspective of the maternal mRNA translation program during oocyte development.Seminars in cell & developmental biology · 2024Review
- The translational oscillation in oocyte and early embryo development.Nucleic acids research · 2023Article
- Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and mOpen biology · 2023Article
- LSM14B is an Oocyte-Specific RNA-Binding Protein Indispensable for Maternal mRNA Metabolism and Oocyte Development in Mice.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- High-resolution ribosome profiling reveals translational selectivity for transcripts in bovine preimplantation embryo development.Development (Cambridge, England) · 2022Article
- Ultrasensitive Ribo-seq reveals translational landscapes during mammalian oocyte-to-embryo transition and pre-implantation development.Nature cell biology · 2022Article
- SGK1 is essential for meiotic resumption in mammalian oocytes.European journal of cell biology · 2022Article
- ncRNA BC1 influences translation in the oocyte.RNA biology · 2021Article
- p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice.Communications biology · 2021Article
- Oocyte specific lncRNA variantNon-coding RNA research · 2021Article
- Article
- Article
- Expression of lamin C2 in mammalian oocytes.PloS one · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Meiotic maturation of oocyte relies on pre-synthesised maternal mRNA, the translation of which is highly coordinated in space and time. Here, we provide a detailed polysome profiling protocol that demonstrates a combination of the sucrose gradient ultracentrifugation in small SW55Ti tubes with the qRT-PCR-based quantification of 18S and 28S rRNAs in fractionated polysome profile. This newly optimised method, named Scarce Sample Polysome Profiling (SSP-profiling), is suitable for both scarce and conventional sample sizes and is compatible with downstream RNA-seq to identify polysome associated transcripts. Utilising SSP-profiling we have assayed the translatome of mouse oocytes at the onset of nuclear envelope breakdown (NEBD)-a developmental point, the study of which is important for furthering our understanding of the molecular mechanisms leading to oocyte aneuploidy. Our analyses identified 1847 transcripts with moderate to strong polysome occupancy, including abundantly represented mRNAs encoding mitochondrial and ribosomal proteins, proteasomal components, glycolytic and amino acids synthetic enzymes, proteins involved in cytoskeleton organization plus RNA-binding and translation initiation factors. In addition to transcripts encoding known players of meiotic progression, we also identified several mRNAs encoding proteins of unknown function. Polysome profiles generated using SSP-profiling were more than comparable to those developed using existing conventional approaches, being demonstrably superior in their resolution, reproducibility, versatility, speed of derivation and downstream protocol applicability.
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