Evidence map›Paper›PMID 32070320›Full record

ArticleBMC ophthalmology2020

Protection of retinal function and morphology in MNU-induced retinitis pigmentosa rats by ALDH2: an in-vivo study.

Weiming Yan, Pan Long, Dongyu Wei, Weihua Yan, Xiangrong Zheng, Guocang Chen, Jiancong Wang, Zuoming Zhang, Tao Chen, Meizhu Chen

Open access · goldAbstract read
In one paragraph

Article in BMC ophthalmology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Weiming YanDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University), Fuzhou, 350025, China.
Pan LongDepartment of Ophthalmology, The West General Hospital of Chinese PLA, Chendu, 610083, China.
Dongyu WeiCenter of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an, 710032, China.
Weihua YanTong'an No.1 High School of Fujian Province, Xiamen, 361100, China.
Xiangrong ZhengDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University), Fuzhou, 350025, China.
Guocang ChenDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University), Fuzhou, 350025, China.
Jiancong WangBeiJing HealthOLight Technology Co. Ltd, Beijing, 10010, China.
Zuoming ZhangCenter of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an, 710032, China. zhangzm@fmmu.edu.cn.
Tao ChenCenter of Clinical Aerospace Medicine, Fourth Military Medical University, Xi'an, 710032, China. baiidtf0506@126.com.
Meizhu ChenDepartment of Ophthalmology, The 900th Hospital of Joint Logistic Support Force, PLA (Clinical Medical College of Fujian Medical University, Dongfang Hospital Affiliated to Xiamen University), Fuzhou, 350025, China. jumychen@126.com.
Air Force Medical University · CNXiamen University · CNFujian Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundRetinitis pigmentosa (RP) is a kind of inherited retinal degenerative diseases characterized by the progressive loss of photoreceptors. RP has been a conundrum without satisfactory countermeasures in clinic until now. Acetaldehyde dehydrogenase 2 (ALDH2), a major enzyme involved in aldehyde detoxification, has been demonstrated to be beneficial for a growing number of human diseases, such as cardiovascular dysfunction, diabetes mellitus and neurodegeneration. However, its protective effect against RP remains unknown. Our study explored the impact of ALDH2 on retinal function and structure in N-methyl-N-nitrosourea (MNU)-induced RP rats.

methodsRats were gavaged with 5 mg/kg Alda-1, an ALDH2 agonist, 5 days before and 3 days after MNU administration. Assessments of retinal function and morphology as well as measurement of specific proteins expression level were conducted.

resultsElectroretinogram recordings showed that Alda-1 administration alleviated the decrease in amplitude caused by MNU, rendering protection of retinal function. Mitigation of photoreceptor degeneration in MNU-treated retinas was observed by optical coherence tomography and retinal histological examination. In addition, Western blotting results revealed that ALDH2 protein expression level was upregulatedwith increased expression of SIRT1 protein after the Alda-1 intervention. Besides, endoplasmic reticulum stress (ERS) was reduced according to the significant downregulation of GRP78 protein, while apoptosis was ameliorated as shown by the decreased expression of PARP1 protein.

conclusionsTogether, our data demonstrated that ALDH2 could provide preservation of retinal function and morphology against MNU-induced RP, with the underlying mechanism at least partly related to the modulation of SIRT1, ERS and apoptosis.

Indexed as

Aldehyde Dehydrogenase, MitochondrialAlkylating AgentsAnimalsBenzamidesBenzodioxolesBlotting, WesternDark AdaptationDisease Models, AnimalElectroretinographyEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsInjections, IntraperitonealMaleMethylnitrosoureaPhotic StimulationPoly (ADP-Ribose) Polymerase-1Aldehyde Dehydrogenase, MitochondrialAldh2 protein, ratAlkylating AgentsBenzamidesBenzodioxolesEndoplasmic Reticulum Chaperone BiPGRP78 protein, ratHeat-Shock ProteinsHSPA5 protein, humanMethylnitrosoureaN-(1,3-benzodioxol-5-ylmethyl)-2,6-dichlorobenzamideParp1 protein, ratPoly (ADP-Ribose) Polymerase-1Sirt1 protein, ratSirtuin 1ALDH2ElectroretinogramEndoplasmic reticulum stressPhotoreceptor degenerationRetinitis pigmentosaSIRT1

Identifiers

PMID32070320
PMCPMC7027227
OpenAlexW3007144856

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.