Evidence map›Paper›PMID 32084157›Full record

ArticlePloS one2020

Serum lipopolysaccharide neutralizing capacity in ischemic stroke.

Jaakko Leskelä, Milla Pietiäinen, Anton Safer, Markku Lehto, Jari Metso, Ernst Malle, Florian Buggle, Heiko Becher, Jouko Sundvall, Armin J Grau and 2 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed, 1 pooled it
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. A Review on the Role of Oral Bacteria in Stroke.International journal of molecular sciences · 2025
    Review
  3. Review
  4. Exosomal miR-155-5p promote the occurrence of carotid atherosclerosis.Journal of cellular and molecular medicine · 2024
    Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 9 institutions in 3 countries.

Jaakko LeskeläOral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.ORCID 0000-0001-9670-339X
Milla PietiäinenOral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.
Anton SaferInstitute of Global Health, University of Heidelberg, Heidelberg, Germany.
Markku LehtoFolkhälsan Institute of Genetics, Folkhälsan Research Center, Helsinki, Finland.
Jari MetsoMinerva Foundation Institute for Medical Research, Helsinki, Finland.
Ernst MalleDivision of Molecular Biology and Biochemistry, Gottfried Schatz Research Center, Medical University of Graz, Graz, Austria.
Florian BuggleDepartment of Neurology, Klinikum Ludwigshafen, Ludwigshafen, Germany.
Heiko BecherUniversity Medical Center Hamburg-Eppendorf, Institute of Medical Biometry and Epidemiology, Hamburg, Germany.
Jouko SundvallNational Institute for Health and Welfare, Helsinki, Finland.
Armin J GrauDepartment of Neurology, Klinikum Ludwigshafen, Ludwigshafen, Germany.
Pirkko J PussinenOral and Maxillofacial Diseases, University of Helsinki, Helsinki, Finland.ORCID 0000-0003-3563-1876
Frederick PalmDepartment of Neurology, Helios Klinikum Schleswig, Schleswig, Germany.
University of Helsinki · FIKlinikum Ludwigshafen · DEFinnish Institute for Health and Welfare · FIFolkhälsans Forskningscentrum · FIHeidelberg University · DEHelios Vogtland Klinikum Plauen · DEMedical University of Graz · ATMinerva Foundation · FIUniversität Hamburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionPeriodontitis is associated with increased serum lipopolysaccharide (LPS) activity, which may be one mechanism linking periodontitis with the risk of cardiovascular diseases. As LPS-carrying proteins including lipoproteins modify LPS-activity, we investigated the determinants of serum LPS-neutralizing capacity (LPS-NC) in ischemic stroke. The association of LPS-NC and Aggregatibacter actinomycetemcomitans, a major microbial biomarker in periodontitis, was also investigated. MATERIALS AND

methodsThe assay to measure LPS-NC was set up by spiking serum samples with E. coli LPS. The LPS-NC, LPS-binding protein (LBP), soluble CD14 (sCD14), lipoprotein profiles, apo(lipoprotein) A-I, apoB, and phospholipid transfer protein (PLTP) activity, were determined in 98 ischemic stroke patients and 100 age- and sex-matched controls. Serum and saliva immune response to A. actinomycetemcomitans, its concentration in saliva, and serotype-distribution were examined.

resultsLPS-NC values ranged between 51-83% in the whole population. Although several of the LPS-NC determinants differed significantly between cases and controls (PLTP, sCD14, apoA-I, HDL-cholesterol), the levels did not (p = 0.056). The main determinants of LPS-NC were i) triglycerides (β = -0.68, p<0.001), and ii) HDL cholesterol (0.260, <0.001), LDL cholesterol (-0.265, <0.001), PLTP (-0.196, 0.011), and IgG against A. actinomycetemcomitans (0.174, 0.011). Saliva A. actinomycetemcomitans concentration was higher [log mean (95% CI), 4.39 (2.35-8.19) vs. 10.7 (5.45-21) genomes/ml, p = 0.023) and serotype D more frequent (4 vs. 0%, p = 0.043) in cases than controls. Serotypeablity or serotypes did not, however, relate to the LPS-NC.

conclusionSerum LPS-NC comprised low PLTP-activity, triglyceride and LDL cholesterol concentrations, as well as high HDL cholesterol and IgG against A. actinomycetemcomitans. The present findings let us to conclude that LPS-NC did not associate with stroke.

Indexed as

AgedBrain IschemiaFemaleHumansLipopolysaccharidesMaleStrokeLipopolysaccharides

Identifiers

PMID32084157
PMCPMC7034831
OpenAlexW3001442764

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.