Evidence mapPaperPMID 32094115Full record

ArticleDevelopment (Cambridge, England)2020

A single cell transcriptome atlas of the developing zebrafish hindbrain.

Monica Tambalo, Richard Mitter, David G Wilkinson

Open access · hybridAbstract read
In one paragraph

Article in Development (Cambridge, England), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 77 citations in OpenAlex.

  1. Article
  2. Modern Mining: The Role of Single-cell RNA Sequencing in Advancing Neuroscience Research.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Monica TambaloNeural Development Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.ORCID 0000-0003-4240-0320
Richard MitterBioinformatics and Biostatistics, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.ORCID 0000-0002-1279-3760
David G WilkinsonNeural Development Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK david.wilkinson@crick.ac.uk.ORCID 0000-0001-6757-7080
The Francis Crick Institute · GB

Funding

Medical Research Council FC001217Wellcome Trust FC001217
6 · The paper itself

Abstract

Segmentation of the vertebrate hindbrain leads to the formation of rhombomeres, each with a distinct anteroposterior identity. Specialised boundary cells form at segment borders that act as a source or regulator of neuronal differentiation. In zebrafish, there is spatial patterning of neurogenesis in which non-neurogenic zones form at boundaries and segment centres, in part mediated by Fgf20 signalling. To further understand the control of neurogenesis, we have carried out single cell RNA sequencing of the zebrafish hindbrain at three different stages of patterning. Analyses of the data reveal known and novel markers of distinct hindbrain segments, of cell types along the dorsoventral axis, and of the transition of progenitors to neuronal differentiation. We find major shifts in the transcriptome of progenitors and of differentiating cells between the different stages analysed. Supervised clustering with markers of boundary cells and segment centres, together with RNA-seq analysis of Fgf-regulated genes, has revealed new candidate regulators of cell differentiation in the hindbrain. These data provide a valuable resource for functional investigations of the patterning of neurogenesis and the transition of progenitors to neuronal differentiation.

Indexed as

ZebrafishAnimalsAnimals, Genetically ModifiedAtlases as TopicBody PatterningCell DifferentiationEmbryo, NonmammalianGene Expression ProfilingGene Expression Regulation, DevelopmentalGene Regulatory NetworksNeural Stem CellsNeurogenesisNeuronsRhombencephalonSingle-Cell AnalysisTissue DistributionDorsoventral patterningFgf signallingHindbrain boundaryHindbrain segmentationNeurogenesisSingle cell RNA sequencing

Identifiers

PMID32094115
PMCPMC7097387
OpenAlexW3008658832

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.