ArticleApplied and environmental microbiology2020
Metagenomics-Guided Discovery of Potential Bacterial Metallothionein Genes from the Soil Microbiome That Confer Cu and/or Cd Resistance.
Article in Applied and environmental microbiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Exploring protein natural diversity in environmental microbiomes with DeepMetagenome.Cell reports methods · 2024Article
- Sequence-based Functional Metagenomics Reveals Novel Natural Diversity of Functional CopA in Environmental Microbiomes.Genomics, proteomics & bioinformatics · 2023Article
- Chromium contamination accentuates changes in the microbiome and heavy metal resistome of a tropical agricultural soil.World journal of microbiology & biotechnology · 2023Article
- Emerging enzyme surface display systems for waste resource recovery.Environmental microbiology · 2023Review
- The Molecular Basis of Acinetobacter baumannii Cadmium Toxicity and Resistance.Applied and environmental microbiology · 2021Article
- Rhizosphere Microbial Communities and Heavy Metals.Microorganisms · 2021Review
- A Single Microbiome Gene Alters Murine Susceptibility to Acute Arsenic Exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Genomic Insights Into Cadmium Resistance of a Newly Isolated, Plasmid-FreeFrontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metallothionein (MT) genes are valuable genetic materials for developing metal bioremediation tools. Currently, a limited number of prokaryotic MTs have been experimentally identified, which necessitates the expansion of bacterial MT diversity. In this study, we conducted a metagenomics-guided analysis for the discovery of potential bacterial MT genes from the soil microbiome. More specifically, we combined resistance gene enrichment through diversity loss, metagenomic mining with a dedicated MT database, evolutionary trace analysis, DNA chemical synthesis, and functional genomic validation to identify novel MTs. Results showed that Cu stress induced a compositional change in the soil microbiome, with an enrichment of metal-resistant bacteria in soils with higher Cu concentrations. Shotgun metagenomic sequencing was performed to obtain the gene pool of environmental DNA (eDNA), which was subjected to a local BLAST search against an MT database for detecting putative MT genes. Evolutional trace analysis led to the identification of 27 potential MTs with conserved cysteine/histidine motifs different from those of known prokaryotic MTs. Following chemical synthesis of these 27 potential MT genes and heterologous expression in
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.