Evidence map›Paper›PMID 32126221›Full record

ArticleMechanisms of ageing and development2020

Quantitative proteomics to study aging in rabbit liver.

Bushra Amin, Katarena I Ford, Renã A S Robinson

Open access · greenAbstract read
In one paragraph

Article in Mechanisms of ageing and development, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Bushra AminDepartment of Chemistry, Vanderbilt University, Nashville, TN, 37235, United States.
Katarena I FordDepartment of Chemistry, Vanderbilt University, Nashville, TN, 37235, United States; Department of Neurology, Vanderbilt University Medical Center, Nashville, TN, 37232, United States; Vanderbilt Memory & Alzheimer's Center, Nashville, TN, 37212, United States; Vanderbilt Institute of Chemical Biology, Nashville, TN, 37232, United States; Vanderbilt Brain Institute, Nashville, TN, 37232, United States.
Renã A S RobinsonDepartment of Chemistry, Vanderbilt University, Nashville, TN, 37235, United States; Department of Neurology, Vanderbilt University Medical Center, Nashville, TN, 37232, United States; Vanderbilt Memory & Alzheimer's Center, Nashville, TN, 37212, United States; Vanderbilt Institute of Chemical Biology, Nashville, TN, 37232, United States; Vanderbilt Brain Institute, Nashville, TN, 37232, United States. Electronic address: rena.as.robinson@vanderbilt.edu.
Vanderbilt University · US

Funding

Tumor Immunology and Microenvironment Research ProgramP30CA068485 · NCI · VANDERBILT UNIVERSITY MEDICAL CENTER · PI Ben Ho Park · 1995 to 2026
$172.8M
Vanderbilt Mouse Metabolic Physiology CenterU24DK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2001 to 2015
$14.9M
Vanderbilt Mouse Metabolic Phenotyping CenterU2CDK059637 · NIDDK · VANDERBILT UNIVERSITY · PI WASSERMAN, DAVID H · 2016 to 2021
$6.3M
Novel Multiplexing Proteomics to Study the Periphery in Alzheimer's DiseaseR01GM117191 · NIGMS · VANDERBILT UNIVERSITY · PI ROBINSON, RENA A. S. · 2016 to 2020
$2.1M
NCI NIH HHS P30 CA068485NIDDK NIH HHS U24 DK059637NIDDK NIH HHS U2C DK059637NIGMS NIH HHS R01 GM117191
6 · The paper itself

Abstract

Aging globally effects cellular and organismal metabolism across a range of mammalian species, including humans and rabbits. Rabbits (Oryctolagus cuniculus are an attractive model system of aging due to their genetic similarity with humans and their short lifespans. This model can be used to understand metabolic changes in aging especially in major organs such as liver where we detected pronounced variations in fat metabolism, mitochondrial dysfunction, and protein degradation. Such changes in the liver are consistent across several mammalian species however in rabbits the downstream effects of these changes have not yet been explored. We have applied proteomics to study changes in the liver proteins from young, middle, and old age rabbits using a multiplexing cPILOT strategy. This resulted in the identification of 2,586 liver proteins, among which 45 proteins had significant p < 0.05) changes with aging. Seven proteins were differentially-expressed at all ages and include fatty acid binding protein, aldehyde dehydrogenase, enoyl-CoA hydratase, 3-hydroxyacyl CoA dehydrogenase, apolipoprotein C3, peroxisomal sarcosine oxidase, adhesion G-protein coupled receptor, and glutamate ionotropic receptor kinate. Insights to how alterations in metabolism affect protein expression in liver have been gained and demonstrate the utility of rabbit as a model of aging.

Indexed as

Gene Expression RegulationModels, BiologicalProteomicsAgingAnimalsLiverMaleProteomeRabbitsProteomeAgingcPILOTEnhanced multiplexingLiverMetabolismOryctolagus cuniculusProteomicsRabbit

Identifiers

PMID32126221
PMCPMC7138690
OpenAlexW3008373594

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.