Evidence map›Paper›PMID 32191305›Full record

ArticleThe Journal of antimicrobial chemotherapy2020

RNA markers for ultra-rapid molecular antimicrobial susceptibility testing in fluoroquinolone-treated Klebsiella pneumoniae.

Xi Yang, Marjan M Hashemi, Nadya Andini, Michelle M Li, Shuzhen Kuang, Karen C Carroll, Tza-Huei Wang, Samuel Yang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of antimicrobial chemotherapy, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 11 citations in OpenAlex.

  1. Bacterial stress responses lower mRNA-protein level correlations.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  2. Metabolism-Based Biomarkers for Rapid Phenotypic Antibiotic Susceptibility Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Xi YangDepartment of Emergency Medicine, Stanford University, Stanford, CA, USA.
Marjan M HashemiDepartment of Emergency Medicine, Stanford University, Stanford, CA, USA.
Nadya AndiniDepartment of Emergency Medicine, Stanford University, Stanford, CA, USA.
Michelle M LiDepartment of Mathematical and Computational Science, Stanford University, Stanford, CA, USA.
Shuzhen KuangDepartment of Biological Sciences, Clemson University, Clemson, SC, USA.
Karen C CarrollDepartment of Pathology, Johns Hopkins University, Baltimore, MD, USA.
Tza-Huei WangDepartment of Mechanical Engineering, Johns Hopkins University, Baltimore, MD, USA.
Samuel YangDepartment of Emergency Medicine, Stanford University, Stanford, CA, USA.
Stanford University · USJohns Hopkins University · USClemson University · US

Funding

Technology development for point-of-care detection and antimicrobial susceptibility testing of Neisseria gonorrhoeaeR01AI138978 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF · 2018 to 2021
$5.1M
A "Culture" Shift: Integrated Bacterial Screening and Antibacterial Susceptibility Test on Microfluidic Digital Array for Bloodstream InfectionsR01AI137272 · NIAID · JOHNS HOPKINS UNIVERSITY · PI WANG, TZA-HUEI JEFF, YANG, SAMUEL · 2018 to 2022
$3.6M
NIAID NIH HHS R01 AI137272NIAID NIH HHS R01 AI138978
6 · The paper itself

Abstract

objectivesTraditional antimicrobial susceptibility testing (AST) is growth dependent and time-consuming. With rising rates of drug-resistant infections, a novel diagnostic method is critically needed that can rapidly reveal a pathogen's antimicrobial susceptibility to guide appropriate treatment. Recently, RNA sequencing has been identified as a powerful diagnostic tool to explore transcriptional gene expression and improve AST.

methodsRNA sequencing was used to investigate the potential of RNA markers for rapid molecular AST using Klebsiella pneumoniae and ciprofloxacin as a model. Downstream bioinformatic analysis was applied for optimal marker selection. Further validation on 11 more isolates of K. pneumoniae was performed using quantitative real-time PCR.

resultsFrom RNA sequencing, we identified RNA signatures that were induced or suppressed following exposure to ciprofloxacin. Significant shifts at the transcript level were observed as early as 10 min after antibiotic exposure. Lastly, we confirmed marker expression profiles with concordant MIC results from traditional culture-based AST and validated across 11 K. pneumoniae isolates. recA, coaA and metN transcripts harbour the most sensitive susceptibility information and were selected as our top markers.

conclusionsOur results suggest that RNA signature is a promising approach to AST development, resulting in faster clinical diagnosis and treatment of infectious disease. This approach is potentially applicable in other models including other pathogens exposed to different classes of antibiotics.

Indexed as

Klebsiella InfectionsKlebsiella pneumoniaeAnti-Bacterial AgentsCiprofloxacinFluoroquinolonesHumansMicrobial Sensitivity TestsRNAAnti-Bacterial AgentsCiprofloxacinFluoroquinolonesRNA

Identifiers

PMID32191305
PMCPMC7303823
OpenAlexW3011717132

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.