Evidence mapPaperPMID 32239434Full record

ArticleJournal of cardiovascular translational research2021

Exendin-4 Protects Against Myocardial Ischemia-Reperfusion Injury by Upregulation of SIRT1 and SIRT3 and Activation of AMPK.

Refaat A Eid, Mashael Mohammed Bin-Meferij, Attalla Farag El-Kott, Samy M Eleawa, Mohamed Samir Ahmed Zaki, Mubarak Al-Shraim, Fahmy El-Sayed, Muhammad Alaa Eldeen, Mahmoud A Alkhateeb, Samah A Alharbi and 2 more

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In one paragraph

Article in Journal of cardiovascular translational research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed, 1 pooled it
6.1field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.

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  18. Forkhead box O1 transcription factor; a therapeutic target for diabetic cardiomyopathy.Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 8 institutions in 3 countries.

Refaat A EidDepartment of Pathology, College of Medicine, King Khalid University, Abha, Saudi Arabia. refaat_eid@yahoo.com.
Mashael Mohammed Bin-MeferijDepartment of Biology, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
Attalla Farag El-KottDepartment of Biology, College of Science, King Khalid University, P.O. 641, Abha, 61421, Saudi Arabia.
Samy M EleawaDepartment of Applied Medical Sciences, College of Health Sciences, PAAET, Shuwaikh, Kuwait.
Mohamed Samir Ahmed ZakiDepartment of Anatomy, College of Medicine, King Khalid University, P.O. 641, Abha, 61421, Saudi Arabia.
Mubarak Al-ShraimDepartment of Pathology, College of Medicine, King Khalid University, Abha, Saudi Arabia.
Fahmy El-SayedDepartment of Pathology, College of Medicine, King Khalid University, Abha, Saudi Arabia.
Muhammad Alaa EldeenBiology Department, Physiology Section, Faculty of Science, Zagazig University, Zagazig, Egypt.
Mahmoud A AlkhateebDepartment of Basic Medical Sciences/College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Samah A AlharbiDepartment of Physiology, College of Medicine, Umm Al-Qura University, Mekkah, Saudi Arabia.
Hussain AlderaDepartment of Basic Medical Sciences/College of Medicine, King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.
Mohammad A KhalilDepartment of Basic Medical Sciences, Faculty of Medicine, King Fahad Medical City, Riyadh, Saudi Arabia.
King Khalid University · SAKing Saud bin Abdulaziz University for Health Sciences · SAZagazig University · EGDamanhour University · EGKing Fahd Medical City · SAPrincess Nourah bint Abdulrahman University · SAPublic Authority for Applied Education and Training · KWUmm al-Qura University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study evaluated if the cardioprotective effect of Exendin-4 against ischemia/reperfusion (I/R) injury in male rats involves modulation of AMPK and sirtuins. Adult male rats were divided into sham, sham + Exendin-4, I/R, I/R + Exendin-4, and I/R + Exendin-4 + EX-527, a sirt1 inhibitor. Exendin-4 reduced infarct size and preserved the function and structure of the left ventricles (LV) of I/R rats. It also inhibited oxidative stress and apoptosis and upregulated MnSOD and Bcl-2 in their infarcted myocardium. With no effect on SIRTs 2/6/7, Exendin-4 activated and upregulated mRNA and protein levels of SIRT1, increased levels of SIRT3 protein, activated AMPK, and reduced the acetylation of p53 and PGC-1α as well as the phosphorylation of FOXO-1. EX-527 completely abolished all beneficial effects of Exendin-4 in I/R-induced rats. In conclusion, Exendin-4 cardioprotective effect against I/R involves activation of SIRT1 and SIRT3. Graphical Abstract Exendin-4 could scavenge free radical directly, upregulate p53, and through upregulation of SIRT1 and stimulating SIRT1 nuclear accumulation. In addition, Exendin-4 also upregulates SIRT3 which plays an essential role in the upregulation of antioxidants, inhibition of reactive oxygen species (ROS) generation, and prevention of mitochondria damage. Accordingly, SIRT1 induces the deacetylation of PGC-1α and p53 and is able to bind p-FOXO-1. This results in inhibition of cardiomyocyte apoptosis through increasing Bcl-2 levels, activity, and levels of MnSOD; decreasing expression of Bax; decreasing cytochrome C release; and improving mitochondria biogenesis through upregulation of Mfn-2.

Indexed as

AcetylationAMP-Activated Protein KinasesAnimalsApoptosisDisease Models, AnimalEnzyme ActivationExenatideForkhead Box Protein O1Glucagon-Like Peptide-1 ReceptorGlucagon-Like Peptide-1 Receptor AgonistsIncretinsMaleMyocardial Reperfusion InjuryMyocytes, CardiacNerve Tissue ProteinsOxidative StressAMP-Activated Protein KinasesExenatideForkhead Box Protein O1Foxo1 protein, ratGlp1r protein, ratGlucagon-Like Peptide-1 ReceptorGlucagon-Like Peptide-1 Receptor AgonistsIncretinsNerve Tissue ProteinsPeroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alphaPpargc1a protein, ratSirt1 protein, ratSIRT3 protein, ratSirtuin 1SirtuinsTp53 protein, ratTumor Suppressor Protein p53Exendin-4HeartIschemiaReperfusionSIRT1

Identifiers

PMID32239434
OpenAlexW3014615892

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.