Evidence map›Paper›PMID 32306155›Full record

ArticleVirus genes2020

Identification of a novel bovine copiparvovirus in pooled fetal bovine serum.

Sally A Baylis, Csaba Miskey, Johannes Blümel, Marco Kaiser, Beatrix Kapusinszky, Eric Delwart

Open access · hybridAbstract read
In one paragraph

Article in Virus genes, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
0.1field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Identification of a novelFrontiers in veterinary science · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Sally A BaylisPaul-Ehrlich-Institut, Langen, Germany. Sally.Baylis@pei.de.
Csaba MiskeyPaul-Ehrlich-Institut, Langen, Germany.
Johannes BlümelPaul-Ehrlich-Institut, Langen, Germany.
Marco KaiserGenExpress Gesellschaft für Proteindesign mbH, Berlin, Germany.
Beatrix KapusinszkyVitalant Research Institute, San Francisco, CA, 94118, USA.
Eric DelwartVitalant Research Institute, San Francisco, CA, 94118, USA.
Paul Ehrlich Institut · DEUniversity of California, San Francisco · USVitalant · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel parvovirus was identified as a cell culture contaminant by metagenomic analysis. Droplet digital PCR (ddPCR) was used to determine viral loads in the cell culture supernatant and further analysis, by ddPCR and DNA sequencing, demonstrated that fetal bovine serum (FBS) used during cell culture was the source of the parvovirus contamination. The FBS contained ~ 50,000 copies of the novel parvovirus DNA per ml of serum. The viral DNA was resistant to DNAse digestion. Near-full length sequence of the novel parvovirus was determined. Phylogenetic analysis demonstrated that virus belongs to the Copiparvovirus genus, being most closely related to bovine parvovirus 2 (BPV2) with 41% identity with the non-structural protein NS1 and 47% identity with the virus capsid protein of BPV2. A screen of individual and pooled bovine sera identified a closely related variant of the novel virus in a second serum pool. For classification purposes, the novel virus has been designated bovine copiparvovirus species 3 isolate JB9 (bocopivirus 3-JB9).

Indexed as

MetagenomicsAnimalsBocavirusCattleFetusGenome, ViralParvoviridae InfectionsParvovirinaeSerum Albumin, BovineSerum Albumin, BovineBovine seraCopiparvovirusParvovirus

Identifiers

PMID32306155
PMCPMC7329774
OpenAlexW3016701088

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.