Evidence map›Paper›PMID 32344610›Full record

ArticleMarine drugs2020

Chitosan Coated Microparticles Enhance Simvastatin Colon Targeting and Pro-Apoptotic Activity.

Nabil A Alhakamy, Usama A Fahmy, Osama A A Ahmed, Giuseppe Caruso, Filippo Caraci, Hani Z Asfour, Muhammed A Bakhrebah, Mohammad N Alomary, Wesam H Abdulaal, Solomon Z Okbazghi and 5 more

Open access · goldAbstract read
In one paragraph

Article in Marine drugs, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.4field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 43 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 7 institutions in 4 countries.

Nabil A AlhakamyDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.ORCID 0000-0002-3826-1519
Usama A FahmyDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.ORCID 0000-0002-6094-5570
Osama A A AhmedDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.ORCID 0000-0002-3204-381X
Giuseppe CarusoOasi Research Institute-IRCCS, Via Conte Ruggero, 73, 94018 Troina (EN), Italy.ORCID 0000-0003-1571-5327
Filippo CaraciOasi Research Institute-IRCCS, Via Conte Ruggero, 73, 94018 Troina (EN), Italy.
Hani Z AsfourDepartment of Medical Microbiology and Parasitology, Faculty of Medicine, Princess Al-Jawhara Center of Excellence in Research of Hereditary Disorders, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Muhammed A BakhrebahLife Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11442, Saudi Arabia.
Mohammad N AlomaryLife Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11442, Saudi Arabia.ORCID 0000-0001-7909-4377
Wesam H AbdulaalDepartment of Biochemistry, Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.ORCID 0000-0002-2510-1946
Solomon Z OkbazghiGlobal Analytical and Pharmaceutical Development, Alexion Pharmaceuticals, New Haven, CT 06510, USA.
Ashraf B Abdel-NaimDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Basma G EidDepartment of Pharmacology and Toxicology, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Hibah M AldawsariDepartment of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Mallesh KurakulaDepartment of Biomedical Engineering, University of Memphis, Memphis, TN 38152, USA.
Amir I MohamedDepartment of Pharmaceutics and Industrial Pharmacy, Military Medical Academy, Cairo 11435, Egypt.
King Abdulaziz University · SAKing Abdulaziz City for Science and Technology · SAAlexion Pharmaceuticals (United States) · USMilitary Medical Academy · EGOasi Maria SS · ITUniversity of Catania · ITUniversity of Memphis · US

Funding

Deanship of Scientific research, King Abdulaziz University (RG-10-166-38).
6 · The paper itself

Abstract

This work aimed at improving the targeting and cytotoxicity of simvastatin (SMV) against colon cancer cells. SMV was encapsulated in chitosan polymers, followed by eudragit S100 microparticles. The release of SMV double coated microparticles was dependent on time and pH. At pH 7.4 maximum release was observed for 6 h. The efficiency of the double coat to target colonic tissues was confirmed using real-time X-ray radiography of iohexol dye. Entrapment efficiency and particle size were used in the characterization of the formula. Cytotoxicity of SMV microparticles against HCT-116 colon cancer cells was significantly improved as compared to raw SMV. Cell cycle analysis by flow cytomeric technique indicated enhanced accumulation of colon cancer cells in the G2/M phase. Additionally, a significantly higher cell fraction was observed in the pre-G phase, which highlighted enhancement of the proapoptotic activity of SMV prepared in the double coat formula. Assessment of annexin V staining was used for confirmation. Cell fraction in early, late and total cell death were significantly elevated. This was accompanied by a significant elevation of cellular caspase 3 activity. In conclusion, SMV-loaded chitosan coated with eudragit S100 formula exhibited improved colon targeting and enhanced cytotoxicity and proapoptotic activity against HCT-116 colon cancer cells.

Indexed as

Drug Delivery SystemsAnimalsAntineoplastic AgentsApoptosisChitosanColonic NeoplasmsDrug CarriersHCT116 CellsHumansHydrogen-Ion ConcentrationMaleMicrospheresParticle SizePolymethacrylic AcidsRabbitsSimvastatinAntineoplastic AgentsChitosanDrug Carriersmethylmethacrylate-methacrylic acid copolymerPolymethacrylic AcidsSimvastatinchitosandrug releasemicroparticlesmucoadhesionsimvastatin

Identifiers

PMID32344610
PMCPMC7231066
OpenAlexW3019078490

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.