ArticlePLoS genetics2020
ArdC, a ssDNA-binding protein with a metalloprotease domain, overpasses the recipient hsdRMS restriction system broadening conjugation host range.
Article in PLoS genetics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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19 citing papers in PubMed, 34 citations in OpenAlex.
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- Pathogen species and capsule expression shape opportunistic transfer and the recipient response during plasmid RP4 conjugation.Communications biology · 2026Article
- BlihIA-A Novel Type I Restriction-Modification System fromInternational journal of molecular sciences · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation.Nucleic acids research · 2024Article
- Cobalt complexes modulate plasmid conjugation in Escherichia coli and Klebsiella pneumoniae.Scientific reports · 2024Article
- Inhibitors of bacterial immune systems: discovery, mechanisms and applications.Nature reviews. Genetics · 2024Review
- Recovering high-quality bacterial genomes from cross-contaminated cultures: a case study of marine Vibrio campbellii.BMC genomics · 2024Article
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- A compendium of viruses from methanogenic archaea reveals their diversity and adaptations to the gut environment.Nature microbiology · 2023Article
- Delineation of a lactococcal conjugation system reveals a restriction-modification evasion system.Microbial biotechnology · 2023Article
- Conjugation's Toolkit: the Roles of Nonstructural Proteins in Bacterial Sex.Journal of bacteriology · 2023Review
- Comparative sequence analysis of pPATH pathogenicity plasmids inFrontiers in plant science · 2023Article
- Five copper homeostasis gene clusters encode the Cu-efflux resistome of the highly copper-tolerantPeerJ · 2023Article
- Anti-Restriction Gene Homologs Are Highly Represented in Methicillin-Resistant and Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Towards a better understanding of antimicrobial resistance dissemination: what can be learnt from studying model conjugative plasmids?Military Medical Research · 2022Review
- Establishment Genes Present on pLS20 Family of Conjugative Plasmids Are Regulated in Two Different Ways.Microorganisms · 2021Article
- Genome archaeology of two laboratory Salmonella enterica enterica sv Typhimurium.G3 (Bethesda, Md.) · 2021Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmids, when transferred by conjugation in natural environments, must overpass restriction-modification systems of the recipient cell. We demonstrate that protein ArdC, encoded by broad host range plasmid R388, was required for conjugation from Escherichia coli to Pseudomonas putida. Expression of ardC was required in the recipient cells, but not in the donor cells. Besides, ardC was not required for conjugation if the hsdRMS system was deleted in P. putida recipient cells. ardC was also required if the hsdRMS system was present in E. coli recipient cells. Thus, ArdC has antirestriction activity against the HsdRMS system and consequently broadens R388 plasmid host range. The crystal structure of ArdC was solved both in the absence and presence of Mn2+. ArdC is composed of a non-specific ssDNA binding N-terminal domain and a C-terminal metalloprotease domain, although the metalloprotease activity was not needed for the antirestriction function. We also observed by RNA-seq that ArdC-dependent conjugation triggered an SOS response in the P. putida recipient cells. Our findings give new insights, and open new questions, into the antirestriction strategies developed by plasmids to counteract bacterial restriction strategies and settle into new hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.