Evidence mapPaperPMID 32352713Full record

Observational studyClinical and translational gastroenterology2020

Epigenetic Alterations Are Associated With Gastric Emptying Disturbances in Diabetes Mellitus.

Susrutha Puthanmadhom Narayanan, Jeong-Heon Lee, Aditya Bhagwate, Saatchi Kuwelker, Huihuang Yan, Tamas Ordog, Adil E Bharucha

Open access · goldAbstract readObservational Study
In one paragraph

Observational study in Clinical and translational gastroenterology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Hyperglycemia-induced increasing ofAnnals of translational medicine · 2021
    Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Susrutha Puthanmadhom NarayananDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA.
Jeong-Heon LeeDivision of Experimental Pathology and Laboratory Medicine, Mayo Clinic, Rochester, Minnesota, USA.
Aditya BhagwateDivision of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, USA.
Saatchi KuwelkerDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA.
Huihuang YanDivision of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, USA.
Tamas OrdogDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA.
Adil E BharuchaDivision of Gastroenterology and Hepatology, Mayo Clinic, Rochester, Minnesota, USA.
Mayo Clinic · USWinnMed · US

Funding

Pathobiology of the Enteric SystemP01DK068055 · MAYO CLINIC · 2004 to 2005
$2.5M
Epigenetic dysregulation in diabetic enteric neuropathyR01DK126827 · NIDDK · MAYO CLINIC ROCHESTER · PI Tamas Ordog · 2022 to 2024
$1.6M
Pilot and Feasibility ProgramP30DK084567 · MAYO CLINIC ROCHESTER · 2025 to 2025
$1.2M
Interstitial Cells of Cajal in Diabetic GastropathyR01DK058185 · UNIVERSITY OF NEVADA RENO · 2002 to 2005
$784k
NIDDK NIH HHS P01 DK068055NIDDK NIH HHS P30 DK084567NIDDK NIH HHS R01 DK058185
6 · The paper itself

Abstract

introductionEpigenetic modifications have been implicated to mediate several complications of diabetes mellitus (DM), especially nephropathy and retinopathy. Our aim was to ascertain whether epigenetic alterations in whole blood discriminate among patients with DM with normal, delayed, and rapid gastric emptying (GE).

methodsUsing the ChIP-seq (chromatin immunoprecipitation combined with next-generation sequencing) assays, we compared the genome-wide enrichment of 3 histone modifications (i.e., H3K4me3, H3K9ac, and H3K27ac) in buffy coats from 20 diabetic patients with gastrointestinal symptoms and normal (n = 6), delayed (n = 8), or rapid (n = 6) GE.

resultsBetween patients with DM with delayed vs normal GE, there were 108 and 54 genes that were differentially bound (false discovery rate < 0.05) with H3K27ac and H3K9ac, respectively; 100 genes were differentially bound with H3K9ac in patients with rapid vs normal GE. The differentially bound genes with H3K27ac were functionally linked to the type 2 immune response, particularly Th2 cell activation and function (e.g., CCR3, CRLF2, CXCR4, IL5RA, and IL1RL1) and glucose homeostasis (FBP-1, PDE4A, and CMKLR1). For H3K9ac, the differentially occupied genes were related to T-cell development and function (e.g., ICOS and CCR3) and innate immunity (RELB, CD300LB, and CLEC2D). Compared with normal GE, rapid GE had differential H3K9ac peaks at the promoter site of diverse immunity-related genes (e.g., TNFRSF25 and CXCR4) and genes related to insulin resistance and glucose metabolism. Motif analysis disclosed enrichment of binding sites for transcription factors relevant to the pathogenesis and complications of DM. DISCUSSION: GE disturbances in DM are associated with epigenetic alterations that pertain to dysimmunity, glucose metabolism, and other complications of DM.

Indexed as

Epigenesis, GeneticAdultBlood Buffy CoatChromatin Immunoprecipitation SequencingComputational BiologyDiabetes Mellitus, Type 1Diabetes Mellitus, Type 2FemaleGastric EmptyingGastrointestinal DiseasesGlucoseHistone CodeHistonesHumansInsulin ResistanceMaleGlucoseHistones

Identifiers

PMID32352713
PMCPMC7145053
OpenAlexW3011559254

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.