Evidence mapPaperPMID 32397533Full record

ArticleInternational journal of molecular sciences2020

Morphometric, Hemodynamic, and Multi-Omics Analyses in Heart Failure Rats with Preserved Ejection Fraction.

Wenxi Zhang, Huan Zhang, Weijuan Yao, Li Li, Pei Niu, Yunlong Huo, Wenchang Tan

Open access · goldAbstract readComparative Study
In one paragraph

Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
2.7field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 28 citations in OpenAlex.

  1. Review
  2. Article
  3. Canagliflozin ameliorates ferritinophagy in HFpEF rats.Journal of geriatric cardiology : JGC · 2025
    Article
  4. Review
  5. Review
  6. Proteomics of the heart.Physiological reviews · 2024
    Review
  7. Article
  8. Review
  9. Article
  10. Article
  11. PINK1 Phosphorylates Drp1International journal of molecular sciences · 2022
    Article
  12. Article
  13. Review
  14. Article
  15. Review
  16. Article
  17. Article
  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Wenxi ZhangDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Huan ZhangHemorheology Center, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Weijuan YaoHemorheology Center, Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China.
Li LiDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Pei NiuDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Yunlong HuoInstitute of Mechanobiology & Medical Engineering, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.
Wenchang TanDepartment of Mechanics and Engineering Science, College of Engineering, Peking University, Beijing 100871, China.
Peking University · CNHong Kong University of Science and Technology · HKShanghai Jiao Tong University · CN

Funding

Leading Talents Program of Guangdong Province 2016LJ06S686National Natural Science Foundation of China 11672006National Natural Science Foundation of China 11732001Shenzhen Science and Technology R&D KQTD20180411143400981
6 · The paper itself

Abstract

(1) Background: There are no successive treatments for heart failure with preserved ejection fraction (HFpEF) because of complex interactions between environmental, histological, and genetic risk factors. The objective of the study is to investigate changes in cardiomyocytes and molecular networks associated with HFpEF. (2) Methods: Dahl salt-sensitive (DSS) rats developed HFpEF when fed with a high-salt (HS) diet for 7 weeks, which was confirmed by in vivo and ex vivo measurements. Shotgun proteomics, microarray, Western blot, and quantitative RT-PCR analyses were further carried out to investigate cellular and molecular mechanisms. (3) Results: Rats with HFpEF showed diastolic dysfunction, impaired systolic function, and prolonged repolarization of myocytes, owing to an increase in cell size and apoptosis of myocytes. Heatmap of multi-omics further showed significant differences between rats with HFpEF and controls. Gene Set Enrichment Analysis (GSEA) of multi-omics revealed genetic risk factors involved in cardiac muscle contraction, proteasome, B cell receptor signaling, and p53 signaling pathway. Gene Ontology (GO) analysis of multi-omics showed the inflammatory response and mitochondrial fission as top biological processes that may deteriorate myocyte stiffening. GO analysis of protein-to-protein network indicated cytoskeleton protein, cell fraction, enzyme binding, and ATP binding as the top enriched molecular functions. Western blot validated upregulated Mff and Itga9 and downregulated Map1lc3a in the HS group, which likely contributed to accumulation of aberrant mitochondria to increase ROS and elevation of myocyte stiffness, and subsequent contractile dysfunction and myocardial apoptosis. (4) Conclusions: Multi-omics analysis revealed multiple pathways associated with HFpEF. This study shows insight into molecular mechanisms for the development of HFpEF and may provide potential targets for the treatment of HFpEF.

Indexed as

ProteomeTranscriptomeAnimalsApoptosisEchocardiographyElectrocardiographyGene OntologyHeart FailureHemodynamicsHumansMaleMitochondria, HeartMyocytes, CardiacRatsRats, Inbred DahlReal-Time Polymerase Chain ReactionProteomeSodium Chloride, Dietaryheart failure with preserved ejection fractionleft ventricular hypertensionmulti-omics analysismyocardial remodelingspeckle tracking echocardiography

Identifiers

PMID32397533
PMCPMC7247709
OpenAlexW3021199580

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.