Evidence map›Paper›PMID 32398044›Full record

ArticleBiology of sex differences2020

Transcriptional analysis of the multiple Sry genes and developmental program at the onset of testis differentiation in the rat.

Jeremy W Prokop, Surya B Chhetri, J Edward van Veen, Xuqi Chen, Adam C Underwood, Katie Uhl, Melinda R Dwinell, Aron M Geurts, Stephanie M Correa, Arthur P Arnold

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology of sex differences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 11 citations in OpenAlex.

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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 6 institutions in 1 country.

Jeremy W ProkopDepartment of Pediatrics and Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI, 49503, USA.
Surya B ChhetriHudsonAlpha Institute for Biotechnology, Huntsville, AL, 35806, USA.
J Edward van VeenDepartment of Integrative Biology & Physiology, Laboratory of Neuroendocrinology of the Brain Research Institute, University of California, 610 Charles Young Drive South, Los Angeles, CA, 90095, USA.
Xuqi ChenDepartment of Integrative Biology & Physiology, Laboratory of Neuroendocrinology of the Brain Research Institute, University of California, 610 Charles Young Drive South, Los Angeles, CA, 90095, USA.
Adam C UnderwoodDepartment of Pediatrics and Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI, 49503, USA.
Katie UhlDepartment of Pediatrics and Human Development, College of Human Medicine, Michigan State University, Grand Rapids, MI, 49503, USA.
Melinda R DwinellDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Aron M GeurtsDepartment of Physiology, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Stephanie M CorreaDepartment of Integrative Biology & Physiology, Laboratory of Neuroendocrinology of the Brain Research Institute, University of California, 610 Charles Young Drive South, Los Angeles, CA, 90095, USA.
Arthur P ArnoldDepartment of Integrative Biology & Physiology, Laboratory of Neuroendocrinology of the Brain Research Institute, University of California, 610 Charles Young Drive South, Los Angeles, CA, 90095, USA. arnold@ucla.edu.ORCID 0000-0002-9908-9767
Medical College of Wisconsin · USAssociation of Research Libraries · USHudsonAlpha Institute for Biotechnology · USJohns Hopkins University · USMichigan State University · USWalsh University · US

Funding

Using a Sequence-to-Structure-to-Function Approach to Functionally Characterize Protein Coding Missense Mutations in the Human and Rat GenomesK01ES025435 · NIEHS · MICHIGAN STATE UNIVERSITY · PI PROKOP, JEREMY WILLIAM · 2014 to 2018
$489k
Sry transgenic rats for investigation of XX vs. XY differences in phenotypesR21OD026560 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ARNOLD, ARTHUR P, DWINELL, MELINDA R · 2018 to 2019
$440k
David Geffen School of Medicine, University of California, Los Angeles (US) noneNIEHS NIH HHS K01 ES025435NIH HHS R21 OD026560
6 · The paper itself

Abstract

backgroundThe commonly used laboratory rat, Rattus norvegicus, is unique in having multiple Sry gene copies found on the Y chromosome, with different copies encoding amino acid variations that influence the resulting protein function. It is not clear which Sry genes are expressed at the onset of testis differentiation or how their expression correlates with that of other genes in testis-determination pathways.

methodsHere, two independent E11-E14 developmental RNAseq datasets show that multiple Sry genes are expressed at E12-E13.

resultsThe identified copies expressed during testis initiation include Sry4A, Sry1, and Sry3C, which are conserved in every strain of Rattus norvegicus with genomes sequenced to date.

conclusionsThis work represents a first step in defining the complex environment of rat testis differentiation that can open the door for generating sex reversal model systems using embryo manipulation techniques that have been available in the mouse but not the rat.

Indexed as

Genes, sryAnimalsGene Expression Regulation, DevelopmentalMaleRats, Sprague-DawleyTestisTranscription, GeneticEmbryoGonadal ridgeRattus norvegicusRNAseqSex determinationSryTestis determinationTranscriptome

Identifiers

PMID32398044
PMCPMC7216353
OpenAlexW3028487811

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.