Evidence map›Paper›PMID 32423650›Full record

ArticleMethods in cell biology2020

Manipulating cultured mammalian cells for mitosis research.

Charles A Day, Alyssa Langfald, Edward H Hinchcliffe

Abstract read
In one paragraph

Article in Methods in cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Inhibition of Collagenase Q1 ofAdvanced therapeutics · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Charles A DayHormel Institute and Masonic Cancer Center, University of Minnesota, Austin, MN, United States.
Alyssa LangfaldHormel Institute and Masonic Cancer Center, University of Minnesota, Austin, MN, United States.
Edward H HinchcliffeHormel Institute and Masonic Cancer Center, University of Minnesota, Austin, MN, United States. Electronic address: hinc0002@umn.edu.

Funding

The functional role of the cPLA2alpha/C1P interaction in sepsis resolutionR01HL125353 · NHLBI · VIRGINIA COMMONWEALTH UNIVERSITY · PI BROWN, RHODERICK E, CHALFANT, CHARLES E. · 2014 to 2018
$4.0M
Research Training Program in Neuro-OncologyT32CA217836 · NCI · MAYO CLINIC ROCHESTER · PI O'NEILL, BRIAN PATRICK, VILE, RICHARD G. · 2018 to 2022
$1.4M
Combinatorial microRNA signaling in melanomaF30CA171713 · NCI · UNIVERSITY OF COLORADO DENVER · PI NELSON-TAYLOR, SARAH K · 2012 to 2016
$151k
Perceived Racism and Cancer Risk Among Latino Immigrant MenF31CA130136 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ORNELAS, INDIA JANE · 2007 to 2008
$59k
NCI NIH HHS F30 CA171713NCI NIH HHS F31 CA130136NCI NIH HHS T32 CA217836NHLBI NIH HHS R01 HL125353
6 · The paper itself

Abstract

The study of mitosis has always relied on bulk-preparation biochemistry techniques (Mazia & Dan, 1952), but very early on lent itself to living, single cell microscopic techniques (Inoue, 1953; Taylor, 1959). Here we describe several of the methods used by our lab to study cell division in living cultured cells, including cold-induced mitotic arrest, cold-induced chromosome missegregation, same-cell live and fixed cell imaging, and microinjection of inactivating antibodies. We detail our imaging system based on an upright fluorescent microscope and spinning disk confocal, as well as the customized "HEKS" metal support slide imaging chambers.

Indexed as

MitosisResearchAnimalsCell Culture TechniquesCell LineChlorocebus aethiopsHumansImaging, Three-DimensionalMammalsMicroinjectionsRatsSpindle ApparatusCell cultureCell cycleChillingChromosomesCold-dependentMicroinjectionMicroscopyMicrotubuleSpindleSpinning disk

Identifiers

PMID32423650
PMCPMC9245630

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.