Evidence map›Paper›PMID 32430322›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2020

Low-frequency ultrasound-mediated cytokine transfection enhances T cell recruitment at local and distant tumor sites.

Tali Ilovitsh, Yi Feng, Josquin Foiret, Azadeh Kheirolomoom, Hua Zhang, Elizabeth S Ingham, Asaf Ilovitsh, Spencer K Tumbale, Brett Z Fite, Bo Wu and 11 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 91 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors at 4 institutions in 2 countries.

Tali IlovitshMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0001-6215-0299
Yi FengMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Josquin FoiretMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0002-7946-8161
Azadeh KheirolomoomMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0002-2173-4893
Hua ZhangMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Elizabeth S InghamDepartment of Biomedical Engineering, University of California, Davis, CA 95616.
Asaf IlovitshMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Spencer K TumbaleMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0002-0616-8280
Brett Z FiteMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Bo WuMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Marina N RaieMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Nisi ZhangMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Aris J KareMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0002-4896-2853
Michael ChavezDepartment of Bioengineering, Stanford University, Stanford, CA 94305.
Lei S QiDepartment of Bioengineering, Stanford University, Stanford, CA 94305.ORCID 0000-0002-3965-3223
Gadi PelledDepartment of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048.ORCID 0000-0003-3857-5531
Dan GazitDepartment of Surgery, Cedars-Sinai Medical Center, Los Angeles, CA 90048.
Ophir VermeshMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Idan SteinbergMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.
Sanjiv S GambhirMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305.ORCID 0000-0002-2711-7554
Katherine W FerraraMolecular Imaging Program at Stanford, Stanford University, Stanford, CA 94305; kwferrar@stanford.edu.ORCID 0000-0002-4976-9107
Stanford University · USUniversity of California, Davis · USCedars-Sinai Medical Center · USXi'an Jiaotong University · CN

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI Gina M Dayton · 2002 to 2026
$84.9M
Translational Oncology Research Program (Project-005)P30CA124435 · NCI · STANFORD UNIVERSITY · PI Vittorio Sebastiano · 2007 to 2026
$71.4M
Supplement to Enhance Wellness and Resiliency in the Graduate EnvironmentT32GM007276 · NIGMS · STANFORD UNIVERSITY · PI MORRISON, ASHBY J. · 1985 to 2023
$32.9M
Specific and high-resolution ultrasound cancer imagingR01CA112356 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI FERRARA, KATHERINE W, FOIRET, JOSQUIN · 2005 to 2022
$6.1M
Large aperture and wideband modular ultrasound arrays for the diagnosis of liver cancerR01CA211602 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI FERRARA, KATHERINE W, TRAHEY, GREGG E. · 2017 to 2021
$3.3M
Image-guided ultrasound therapy and drug delivery in pancreatic cancerR01CA210553 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI FERRARA, KATHERINE W · 2016 to 2020
$3.2M
The Impact of FUS-Mediated Brain Cancer Therapy on BBB Transport, Cytokines, and Immunocyte TraffickingR01CA227687 · NCI · STANFORD UNIVERSITY · PI BUTTS-PAULY, KIM · 2018 to 2022
$3.1M
Ultrasound-guided DNA delivery for regenerative medicineR01EB026094 · NIBIB · CEDARS-SINAI MEDICAL CENTER · PI GAZIT, DAN · 2018 to 2021
$2.8M
Optimized ultrasound-enhanced immunotherapyR01CA199658 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI FERRARA, KATHERINE W · 2015 to 2019
$2.5M
EXTRAMURAL RESEARCH FACILITIES CONSTRUCTIONC06RR012088 · NCRR · UNIVERSITY OF CALIFORNIA DAVIS · PI CURRY, FITZ-ROY E · 1996 to 1996
–
NCI NIH HHS P30 CA093373NCI NIH HHS R01 CA112356NCI NIH HHS R01 CA199658NCI NIH HHS R01 CA210553NCI NIH HHS R01 CA211602NCI NIH HHS R01 CA227687NCRR NIH HHS C06 RR012088NIBIB NIH HHS R01 EB026094NIGMS NIH HHS T32 GM007276
6 · The paper itself

Abstract

Robust cytotoxic T cell infiltration has proven to be difficult to achieve in solid tumors. We set out to develop a flexible protocol to efficiently transfect tumor and stromal cells to produce immune-activating cytokines, and thus enhance T cell infiltration while debulking tumor mass. By combining ultrasound with tumor-targeted microbubbles, membrane pores are created and facilitate a controllable and local transfection. Here, we applied a substantially lower transmission frequency (250 kHz) than applied previously. The resulting microbubble oscillation was significantly enhanced, reaching an effective expansion ratio of 35 for a peak negative pressure of 500 kPa in vitro. Combining low-frequency ultrasound with tumor-targeted microbubbles and a DNA plasmid construct, 20% of tumor cells remained viable, and ∼20% of these remaining cells were transfected with a reporter gene both in vitro and in vivo. The majority of cells transfected in vivo were mucin 1

Indexed as

Ultrasonic WavesAnimalsCell Line, TumorCell MembraneCell MovementHumansImmunotherapyInterferon-betaMiceMicrobubblesNeoplasms, ExperimentalT-LymphocytesTransfectionInterferon-betamicrobubbletransfectionultrasound

Identifiers

PMID32430322
PMCPMC7293655
OpenAlexW3026471270

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.