Evidence map›Paper›PMID 32475417›Full record

ArticlePoultry science2020

Comparative transcriptome analysis digs out genes related to antifreeze between fresh and frozen-thawed rooster sperm.

Xiao-Long Qi, Kai Xing, Zhen Huang, Yu Chen, Liang Wang, Li-Chang Zhang, Xi-Hui Sheng, Xiang-Guo Wang, He-Min Ni, Yong Guo

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 15 citations in OpenAlex.

  1. Current Applications and Future Prospects of High-ThroughputAnimals : an open access journal from MDPI · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Xiao-Long QiAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Kai XingAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Zhen HuangEcological Construction Department, Beijing Monitoring Station for Animal Husbandry Environment, Beijing 102200, China.
Yu ChenDepartment of Livestock and Poultry Products Testing, Beijing General Station of Animal Husbandry, Beijing 100107, China.
Liang WangDepartment of Livestock and Poultry Products Testing, Beijing General Station of Animal Husbandry, Beijing 100107, China.
Li-Chang ZhangBeijing Foreign Enterprise Service Group Co., Ltd, Beijing Er Shang Group, Beijing 100053, China.
Xi-Hui ShengAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Xiang-Guo WangAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
He-Min NiAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.
Yong GuoAnimal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China. Electronic address: y63guo@126.com.
Beijing University of Agriculture · CNNational Animal Husbandry Service · CNBeijing Municipal Ecological and Environmental Monitoring Center · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to investigate differences in mRNA expression between fresh and frozen-thawed sperm in roosters. In trial 1, gene expression profiles were measured using microarray with Affymetrix GeneChip Chicken Genome Arrays. The results showed that 2,115 genes were differentially expressed between the 2 groups. Among these genes, 2,086 were significantly downregulated and 29 were significantly upregulated in the frozen-thawed sperm group. Gene Ontology (GO) analysis showed that more than 1,000 differentially expressed genes (DEG) of all significantly regulated genes were involved in GO terms including biological processes, molecular function, and cellular component. Kyoto Encyclopedia of Genes and Genomes analysis showed that DEG were significantly (P < 0.05) enriched on ribosome, oxidative phosphorylation, proteasome, cell cycle, oocyte meiosis, and spliceosome pathways. In trial 2, ejaculated semen was collected from 18 roosters and divided into 5 recombinant HSP90 protein-supplemented groups (0.01, 0.1, 0.5, 1, or 2 μg/mL) and one control group with no recombinant HSP90 protein supplementation to evaluate the effect of recombinant HSP90 protein in the extender on post-thaw quality of rooster semen. The results showed that post-thaw sperm viability and motility was significantly improved (P < 0.05) in the extender containing 0.5 and 1 μg/mL of recombinant HSP90 protein compared with the control. Our preliminary results will provide a valuable basis for understanding the potential molecular mechanisms of cryodamage in frozen-thawed sperm and theoretical guidance to improve the fertility of frozen-thawed chicken sperm.

Indexed as

TranscriptomeAnimalsChickensCryoprotective AgentsFreezingGene Expression ProfilingMaleSemen PreservationSpermatozoaCryoprotective Agentsfresh and frozen–thawed spermrecombinant HSP90 proteinroostertranscriptome

Identifiers

PMID32475417
PMCPMC7597663
OpenAlexW3012266013

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.