Evidence map›Paper›PMID 32488069›Full record

ArticleNature communications2020

Human beige adipocytes for drug discovery and cell therapy in metabolic diseases.

Amar M Singh, Liang Zhang, John Avery, Amelia Yin, Yuhong Du, Hui Wang, Zibo Li, Haian Fu, Hang Yin, Stephen Dalton

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed, 1 pooled it
5.3field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 1 synthesis or guideline pooled it, 83 citations in OpenAlex.

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  18. Scalable Generation of Pre-Vascularized and Functional Human Beige Adipose Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Amar M Singh *Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA.
Liang Zhang *Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA.
John Avery *Department of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA.
Amelia YinDepartment of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA.
Yuhong DuDepartment of Pharmacology and Chemical Biology, Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA, 30322, USA.
Hui WangDepartment of Radiology, Biomedical Research Imaging Center, University of North Carolina Chapel Hill, Chapel Hill, NC, 27599, USA.
Zibo LiDepartment of Radiology, Biomedical Research Imaging Center, University of North Carolina Chapel Hill, Chapel Hill, NC, 27599, USA.ORCID http://orcid.org/0000-0002-1063-0298
Haian FuDepartment of Pharmacology and Chemical Biology, Emory Chemical Biology Discovery Center, Emory University School of Medicine, Atlanta, GA, 30322, USA.ORCID http://orcid.org/0000-0002-2362-7979
Hang YinDepartment of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA.ORCID http://orcid.org/0000-0002-9621-0138
Stephen DaltonDepartment of Biochemistry and Molecular Biology, Center for Molecular Medicine, University of Georgia, Athens, GA, 30602, USA. sdalton@uga.edu.ORCID http://orcid.org/0000-0002-3450-1263
University of Georgia · USEmory University · USUniversity of North Carolina at Chapel Hill · US

Funding

Impacts of hypoxia and hypoxia-induced factors on skeletal muscle repair and muscle stem cellsR01AR070178 · NIAMS · UNIVERSITY OF GEORGIA · PI YIN, HANG · 2016 to 2020
$1.6M
Small Animal PET/CT for Preclinical Imaging ResearchS10OD023611 · OD · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LI, ZIBO · 2017 to 2017
$835k
NIAMS NIH HHS R01 AR070178NIH HHS S10 OD023611
6 · The paper itself

Abstract

Human beige adipocytes (BAs) have potential utility for the development of therapeutics to treat diabetes and obesity-associated diseases. Although several reports have described the generation of beige adipocytes in vitro, their potential utility in cell therapy and drug discovery has not been reported. Here, we describe the generation of BAs from human adipose-derived stem/stromal cells (ADSCs) in serum-free medium with efficiencies >90%. Molecular profiling of beige adipocytes shows them to be similar to primary BAs isolated from human tissue. In vitro, beige adipocytes exhibit uncoupled mitochondrial respiration and cAMP-induced lipolytic activity. Following transplantation, BAs increase whole-body energy expenditure and oxygen consumption, while reducing body-weight in recipient mice. Finally, we show the therapeutic utility of BAs in a platform for high-throughput drug screening (HTS). These findings demonstrate the potential utility of BAs as a cell therapeutic and as a tool for the identification of drugs to treat metabolic diseases.

Indexed as

Adipocytes, BeigeAnimalsBody WeightCell- and Tissue-Based TherapyDrug DiscoveryDrug Evaluation, PreclinicalEnergy MetabolismFemaleHigh-Throughput Screening AssaysHumansMaleMesenchymal Stem CellsMetabolic DiseasesMiceMice, Inbred NODMice, SCID

Identifiers

PMID32488069
PMCPMC7265435
OpenAlexW3029176268

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.