ArticleAMB Express2020
Genome sequencing and analysis of fungus Hirsutella sinensis isolated from Ophiocordyceps sinensis.
Article in AMB Express, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
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- Three-Dimensional Structural Heteromorphs of Mating-Type Proteins inJournal of fungi (Basel, Switzerland) · 2025Article
- Prevention of Muscle Atrophy by Low-Molecular-Weight Fraction fromCurrent issues in molecular biology · 2024Article
- Combined metabolome and transcriptome analysis reveal the mechanism of water stress in Ophiocordyceps sinensis.BMC genomics · 2024Article
- Translation of Mutant Repetitive Genomic Sequences inInternational journal of molecular sciences · 2024Article
- Establishment of anCurrent issues in molecular biology · 2024Article
- Mutations and Differential Transcription of Mating-Type and Pheromone Receptor Genes inBiology · 2024Article
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- Unraveling the Secrets of a Double-Life Fungus by Genomics:Journal of fungi (Basel, Switzerland) · 2023Article
- Systematic analyses with genomic and metabolomic insights reveal a new species,Frontiers in microbiology · 2023Article
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- Isolation of strains and their genome sequencing to analyze the mating system of Ophiocordyceps robertsii.PloS one · 2023Article
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- The transcriptome of the entomopathogenic fungusPeerJ · 2023Article
- The complete mitochondrial genome ofMitochondrial DNA. Part B, Resources · 2022Article
- An Overview of Genomics, Phylogenomics and Proteomics Approaches in Ascomycota.Life (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Ophiocordyceps sinensis has been used as a traditional medicine or healthy food in China for thousands of years. Hirsutella sinensis was reported as the only correct anamorph of O. sinensis. It is reported that the laboratory-grown H. sinensis mycelium has similar clinical efficacy and less associated toxicity compared to the wild O. sinensis. The research of the H. sinensis is becoming more and more important and urgent. To gain deeper insight into the biological and pharmacological mechanisms, we sequenced the genome of H. sinensis. The genome of H. sinensis (102.72 Mb) was obtained for the first time, with > 99% coverage. 10,200 protein-encoding genes were predicted based on the genome sequence. A detailed secondary metabolism analysis and structure verification of the main ingredients were performed, and the biosynthesis pathways of seven ingredients (mannitol, cordycepin, purine nucleotides, pyrimidine nucleotides, unsaturated fatty acid, cordyceps polysaccharide and sphingolipid) were predicted and drawn. Furthermore, infection process and mechanism of H. sinensis were studied and elaborated in this article. The enzymes involved in the infection mechanism were also predicted, cloned and expressed to verify the mechanism. The genes and proteins were predicted and annotated based on the genome sequence. The pathways of several active components in H. sinensis were predicted and key enzymes were confirmed. The work presented here would improve the understanding of the genetic basis of this organism, and contribute to further research, production and application of H. sinensis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.