ArticleThe Journal of general physiology2020
Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters.
Article in The Journal of general physiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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Who cites it
111 citing papers in PubMed, 166 citations in OpenAlex.
- Endothelial Piezo1 Hyperactivity Drives Neurovascular Dysfunction in Hypertension.Circulation research · 2026Article
- Recursive feedback between Piezo1 conformation and membrane mechanics drives self-organization into finite clusters.bioRxiv : the preprint server for biology · 2026Article
- Piezo1 in Peripheral and Central Sensitisation: Implications for Chronic Pain.Bioengineering (Basel, Switzerland) · 2026Review
- Cold-gated potentiation of mechanotransduction supports touch sensation below 5°C in a mammalian hibernator.Current biology : CB · 2026Article
- Piezo1 in digestive homeostasis and disease: Barrier function, immune signalling, cellular injury and metabolism.Clinical and translational medicine · 2026Review
- Article
- A two-step clockwork mechanism opens a proteo-lipidic pore in PIEZO2.Nature chemical biology · 2026Article
- Intravital Depletion of Cholesterol Reduces Membrane Dynamics and Increases Mechanosensitivity in Osteocytes In Vivo.bioRxiv : the preprint server for biology · 2026Article
- Calpains as Potential Regulators of Microtubule-Associated Cytokinesis: A Cross-Eukaryotic Unifying Hypothesis.Life (Basel, Switzerland) · 2026Article
- Dynamic regulation of TASK-1 channels under cellular stress.The journal of physiological sciences : JPS · 2026Article
- Lipid composition and mechanical force underlie multi-modal regulation of Piezo1 gating.Science advances · 2026Article
- Deoxy-Piezo1 hyperactivity elevates pump-leak fluxes and lactate production in sickle cells.Biophysical journal · 2026Article
- CholBindNet as an interpretable neural network for cholesterol-binding site classification.Communications chemistry · 2026Article
- Amoeboid cancer cells at a glance.Journal of cell science · 2026Review
- Proton radiation impairs mechanotransduction in C2C12 myoblasts.NPJ microgravity · 2026Article
- Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.Biomolecules · 2026Article
- PIEZO1 Channels Modulate the Small Extracellular Vesicle Release in C2C12 Cells.Journal of cellular physiology · 2026Article
- Lipid Regulation of Mechanosensitive Ion Channels.International journal of molecular sciences · 2026Review
- TMEM63 proteins act as mechanically activated cholesterol modulated lipid scramblases contributing to membrane mechano-resilience.Nature communications · 2026Article
- Choice of lipid supplementation for in vitro erythroid cell culture impacts reticulocyte yield and characteristics.Scientific reports · 2026Article
51 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human mechanosensitive ion channel PIEZO1 is gated by membrane tension and regulates essential biological processes such as vascular development and erythrocyte volume homeostasis. Currently, little is known about PIEZO1 plasma membrane localization and organization. Using a PIEZO1-GFP fusion protein, we investigated whether cholesterol enrichment or depletion by methyl-β-cyclodextrin (MBCD) and disruption of membrane cholesterol organization by dynasore affects PIEZO1-GFP's response to mechanical force. Electrophysiological recordings in the cell-attached configuration revealed that MBCD caused a rightward shift in the PIEZO1-GFP pressure-response curve, increased channel latency in response to mechanical stimuli, and markedly slowed channel inactivation. The same effects were seen in native PIEZO1 in N2A cells. STORM superresolution imaging revealed that, at the nanoscale, PIEZO1-GFP channels in the membrane associate as clusters sensitive to membrane manipulation. Both cluster distribution and diffusion rates were affected by treatment with MBCD (5 mM). Supplementation of polyunsaturated fatty acids appeared to sensitize the PIEZO1-GFP response to applied pressure. Together, our results indicate that PIEZO1 function is directly dependent on the membrane composition and lateral organization of membrane cholesterol domains, which coordinate the activity of clustered PIEZO1 channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.