Evidence map›Paper›PMID 32703108›Full record

ArticleTissue engineering. Part A2021

Collagen-Supplemented Incubation Rapidly Augments Mechanical Property of Fibroblast Cell Sheets.

Yuanjia Zhu, Akshara D Thakore, Justin M Farry, Jinsuh Jung, Shreya Anilkumar, Hanjay Wang, Annabel M Imbrie-Moore, Matthew H Park, Nicholas A Tran, Yi-Ping Joseph Woo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Tissue engineering. Part A, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Yuanjia ZhuDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Akshara D ThakoreDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Justin M FarryDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Jinsuh JungDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Shreya AnilkumarDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Hanjay WangDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Annabel M Imbrie-MooreDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Matthew H ParkDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Nicholas A TranDepartment of Cardiothoracic Surgery, and Stanford University, Stanford, California, USA.
Yi-Ping Joseph WooDepartment of Bioengineering, Stanford University, Stanford, California, USA.
Stanford University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell sheet technology using UpCell™ (Thermo Fisher Scientific, Roskilde, Denmark) plates is a modern tool that enables the rapid creation of single-layered cells without using extracellular matrix (ECM) enzymatic digestion. Although this technique has the advantage of maintaining a sheet of cells without needing artificial scaffolds, these cell sheets remain extremely fragile. Collagen, the most abundant ECM component, is an attractive candidate for modulating tissue mechanical properties given its tunable property. In this study, we demonstrated rapid mechanical property augmentation of human dermal fibroblast cell sheets after incubation with bovine type I collagen for 24 h on UpCell plates. We showed that treatment with collagen resulted in increased collagen I incorporation within the cell sheet without affecting cell morphology, cell type, or cell sheet quality. Atomic force microscopy measurements for controls, and cell sheets that received 50 and 100 μg/mL collagen I treatments revealed an average Young's modulus of their respective intercellular regions: 6.6 ± 1.0, 14.4 ± 6.6, and 19.8 ± 3.8 kPa during the loading condition, and 10.3 ± 4.7, 11.7 ± 2.2, and 18.1 ± 3.4 kPa during the unloading condition. This methodology of rapid mechanical property augmentation of a cell sheet has a potential impact on cell sheet technology by improving the ease of construct manipulation, enabling new translational tissue engineering applications.

Indexed as

CollagenTissue EngineeringAnimalsCattleElastic ModulusExtracellular MatrixFibroblastsHumansCollagenatomic force microscopycell sheetYoung's modulus

Identifiers

PMID32703108
PMCPMC7984934
OpenAlexW3045262850

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.