ArticleInternational journal of hematology2020
A high-throughput detection method for the clonality of Human T-cell leukemia virus type-1-infected cells in vivo.
Article in International journal of hematology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 16 citations in OpenAlex.
- Clonality analysis of HTLV-1-infected cells enhances prognostic precision in smoldering adult T-cell leukemia/ lymphoma.Haematologica · 2025Article
- Advances in clonality analysis of human T-cell leukemia virus type 1-infected cells: from Southern blotting to high-throughput sequencing.Journal of clinical and experimental hematopathology : JCEH · 2025Review
- Performance evaluation of an improved RAISING method for clonality analysis of bovine leukemia virus-infected cells: a collaborative study in Japan.The Journal of veterinary medical science · 2025Article
- Methodological landscape in the field of integration site identification of retroviruses and retroviral vectors.Frontiers in bioengineering and biotechnology · 2025Review
- Clone Dynamics and Its Application for the Diagnosis of Enzootic Bovine Leukosis.Journal of virology · 2023Article
- RAISING is a high-performance method for identifying random transgene integration sites.Communications biology · 2022Article
- A Methodology for Assessing Tumor Clonality of Adult T Cell Leukemia/Lymphoma.Molecular therapy. Methods & clinical development · 2020Article
Corrections and comments
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Authors and funding
22 authors at 7 institutions in 1 country.
Funding
Abstract
Approximately 10-20 million of Human T-cell leukemia virus type-1 (HTLV-1)-infected carriers have been previously reported, and approximately 5% of these carriers develop adult T-cell leukemia/lymphoma (ATL) with a characteristic poor prognosis. In Japan, Southern blotting has long been routinely performed for detection of clonally expanded ATL cells in vivo, and as a confirmatory diagnostic test for ATL. However, alternative methods to Southern blotting, such as sensitive, quantitative, and rapid analytical methods, are currently required in clinical practice. In this study, we developed a high-throughput method called rapid amplification of integration site (RAIS) that could amplify HTLV-1-integrated fragments within 4 h and detect the integration sites in > 0.16% of infected cells. Furthermore, we established a novel quantification method for HTLV-1 clonality using Sanger sequencing with RAIS products, and the validity of the quantification method was confirmed by comparing it with next-generation sequencing in terms of the clonality. Thus, we believe that RAIS has a high potential for use as an alternative routine molecular confirmatory test for the clonality analysis of HTLV-1-infected cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.