Evidence map›Paper›PMID 32735017›Full record

ArticleBioscience reports2020

Knockdown of TRIM52 alleviates LPS-induced inflammatory injury in human periodontal ligament cells through the TLR4/NF-κB pathway.

Peng Liu, Lijun Cui, Lifang Shen

Open access · goldAbstract read
In one paragraph

Article in Bioscience reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 18 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Review
  6. Enamel Matrix Derivative Decreases Pyroptosis-Related Genes in Macrophages.International journal of molecular sciences · 2022
    Article
  7. Article
  8. Article
  9. Article
  10. Pyroptosis-Mediated Periodontal Disease.International journal of molecular sciences · 2021
    Review
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Peng LiuDepartment of Prosthodontics, Shanxi Provincial People's Hospital, Taiyuan, Shanxi Province, China.
Lijun CuiDepartment of Stomatology, Cui Lijun Dental Clinic, Datong, Shanxi Province, China.
Lifang ShenDepartment of Stomatology, Meiyuan Stomatological Hospital, Datong, Shanxi Province, China.
Jinan Stomatological Hospital · CNShanxi Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tripartite motif-containing (TRIM) 52 (TRIM52) is a vital regulator of inflammation. However, the function and mechanisms of TRIM52 in lipopolysaccharide (LPS)-induced inflammatory injury of human periodontal ligament cells (HPDLCs) in periodontitis remain undefined. In the present research, gene expression was determined using a quantitative polymerase chain reaction and Western blot. The effect of TRIM52 on LPS-induced inflammatory injury was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and enyzme-linked immunosorbent assay (ELISA). We found that TRIM52 expression was up-regulated in LPS-treated HPDLCs. Knockdown of TRIM52 alleviated LPS-induced proliferative inhibition and apoptosis promotion in HPDLCs, as evidenced by a decrease in cleaved caspase-3 expression and caspase-3 activity. Silencing TRIM52 suppressed LPS-induced inflammatory response of HPDLCs, as indicated by the decrease in interleukin (IL)-6, IL-8, tumor necrosis factor-α (TNF-α) levels, and increase in IL-10 levels. TRIM52 knockdown inhibited LPS-induced activation of TLR4/nuclear factor-κ B (NF-κB) signaling pathway. Taken together, knockdown of TRIM52 mitigated LPS-induced inflammatory injury via the TLR4/NF-κB signaling pathway, providing an effective therapeutic target for periodontitis.

Indexed as

RNA InterferenceApoptosisApoptosis Regulatory ProteinsCell ProliferationCells, CulturedCytokinesDown-RegulationHumansLipopolysaccharidesNF-kappa BPeriodontal LigamentPeriodontitisSignal TransductionToll-Like Receptor 4Tripartite Motif ProteinsApoptosis Regulatory ProteinsCytokinesLipopolysaccharidesNF-kappa BTLR4 protein, humanToll-Like Receptor 4TRIM52 protein, humanTripartite Motif Proteinsnuclear factor-kappa Bperiodontitistoll like receptor 4tripartite motif containing 52

Identifiers

PMID32735017
PMCPMC7418211
OpenAlexW3046353178

What Socratic holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.