Evidence map›Paper›PMID 32736543›Full record

ArticleBMC pregnancy and childbirth2020

Evaluation of highly sensitive diagnostic tools for the detection of P. falciparum in pregnant women attending antenatal care visits in Colombia.

A M Vásquez, G Vélez, A Medina, E Serra-Casas, A Campillo, I J Gonzalez, S C Murphy, A M Seilie, X C Ding, A Tobón Castaño

2 registry-linked trialsOpen access · goldAbstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC pregnancy and childbirth, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to 2 registered trials, which are not on this map. Cited by 17 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed, 5 pooled it
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03172221 completednot on this map

Clinical Performance of the Histidine Rich Protein 2 (HRP2) Highly Sensitive Rapid Diagnostic Test (HS-RDT) for Malaria Diagnosis in Pregnant Women

TypeobservationalSponsorFoundation for Innovative New Diagnostics, SwitzerlandRan2017 to 2018Enrolled880ConditionsPlasmodium Falciparum, Pregnancy Malaria, Diagnoses DiseaseArmsHS-RDT
NCT05757167 phase4active not recruitingnot on this mapstarted 2023, after this paper: background citation

Improving Neonatal Health Through Rapid Malaria Testing in Early Pregnancy With High-Sensitivity

TypeinterventionalSponsorDuke UniversityRan2023 to 2026Enrolled2,174ConditionsMalaria,Falciparum, Malaria in Pregnancy, Malaria in Childbirth, PregnancyArmsMalaria High-Sensitivity Rapid Diagnostic Test (HS-RDT), Artemether-lumefantrine (AL)
3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 5 syntheses or guidelines pooled it, 26 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 3 countries.

A M VásquezGrupo Malaria, Facultad de Medicina, Universidad de Antioquia, Carrera 53 No. 61-30, Lab 610, Medellín, Colombia. amaria.vasquez@udea.edu.co.
G VélezGrupo Malaria, Facultad de Medicina, Universidad de Antioquia, Carrera 53 No. 61-30, Lab 610, Medellín, Colombia.
A MedinaGrupo Malaria, Facultad de Medicina, Universidad de Antioquia, Carrera 53 No. 61-30, Lab 610, Medellín, Colombia.
E Serra-CasasIndependent Consultant, FIND, Geneva, Switzerland.
A CampilloFIND, Geneva, Switzerland.
I J GonzalezFIND, Geneva, Switzerland.
S C MurphyMalaria Molecular Diagnostic Laboratory, Departments of Laboratory Medicine and Microbiology and the Center for Emerging and Re-emerging Infectious Diseases, 750 Republican St, Seattle, WA, 98109, USA.
A M SeilieMalaria Molecular Diagnostic Laboratory, Departments of Laboratory Medicine and Microbiology and the Center for Emerging and Re-emerging Infectious Diseases, 750 Republican St, Seattle, WA, 98109, USA.
X C DingFIND, Geneva, Switzerland.
A Tobón CastañoGrupo Malaria, Facultad de Medicina, Universidad de Antioquia, Carrera 53 No. 61-30, Lab 610, Medellín, Colombia.
Foundation for Innovative New Diagnostics · CHUniversidad de Antioquia · CONational Center for Emerging and Zoonotic Infectious Diseases · US

Funding

Bill and Melinda Gates Foundation OPP1169555
6 · The paper itself

Abstract

backgroundIn low transmission settings early diagnosis is the main strategy to reduce adverse outcomes of malaria in pregnancy; however, microscopy and rapid diagnostic tests (RDTs) are inadequate for detecting low-density infections. We studied the performance of the highly sensitive-RDT (hsRDT) and the loop mediated isothermal DNA amplification (LAMP) for the detection of P. falciparum in pregnant women.

methodsA cross-sectional study was conducted in two malaria-endemic municipalities in Colombia. We screened pregnant women in the context of an antenatal care program in health facilities and evaluated five tests (microscopy, conventional RDT, hsRDT, LAMP and nested polymerase chain reaction-PCR) for the detection of P. falciparum in peripheral blood, using a quantitative reverse transcription PCR (qRT-PCR) as the reference standard. Diagnostic performance of hsRDT and LAMP were compared with routine testing.

resultsThe prevalence of P. falciparum was 4.5% by qRT-PCR, half of those infections were subpatent. The sensitivity of the hsRDT (64.1%) was slightly better compared to microscopy and cRDT (59 and 53.8% respectively). LAMP had the highest sensitivity (89.7%) for detecting P. falciparum and the ability to detect very low-density infections (minimum parasite density detected 0.08 p/μL).

conclusionsThere is an underestimation of Plasmodium spp. infections by tests routinely used in pregnant women attending antenatal care visits. LAMP methodology can be successfully implemented at local hospitals in malaria-endemic areas. The relevance of detecting and treating this sub-patent P. falciparum infections in pregnant women should be evaluated.

trial registrationClinicalTrials.gov, Identifier: NCT03172221 , Date of registration: May 29, 2017.

Indexed as

AdultColombiaCross-Sectional StudiesFemaleHumansMalaria, FalciparumMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPlasmodium falciparumPregnancyPregnancy Complications, ParasiticPrenatal CareReal-Time Polymerase Chain ReactionSensitivity and SpecificityYoung AdultDiagnosticsLoop mediated isothermal DNA amplification (LAMP)Malaria in pregnancyMicroscopyNucleic acid amplification techniquesRapid diagnostic test

Identifiers

PMID32736543
PMCPMC7393871
OpenAlexW3046285224

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.