Evidence map›Paper›PMID 32758644›Full record

ArticleMicrobes and infection

Characterization of peripheral blood mononuclear cells gene expression profiles of pediatric Staphylococcus aureus persistent and non-carriers using a targeted assay.

Elisabeth Israelsson, Damien Chaussabel, Rebecca S B Fischer, Heather C Moore, D Ashley Robinson, Jesse W Dunkle, Heather T Essigmann, Sharron Record, Eric L Brown

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbes and infection. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Elisabeth IsraelssonDepartment of Systems Immunology, Benaroya Research Institute at Virginia Mason, Seattle, WA, USA.
Damien ChaussabelSystems Biology Department, Sidra Medical and Research Center, Doha, Qatar.
Rebecca S B FischerTexas A&M Health Science Center School of Public Health, Department of Epidemiology and Biostatistics, College Station, TX, USA.
Heather C MooreBaylor College of Medicine, Complex Care Clinic, Texas Children's Hospital, Houston, TX, USA.
D Ashley RobinsonDepartment of Microbiology and Immunology, University of Mississippi Medical Center, Jackson, MS, USA.
Jesse W DunkleIcahn School of Medicine, Mount Sinai Hospital, Institute for Advanced Medicine, New York, NY, USA.
Heather T EssigmannDivision of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas Health Science Center, Houston, TX, USA.
Sharron RecordTexas Children's Hospital, Department of Pediatrics, TX, USA.
Eric L BrownDivision of Epidemiology, Human Genetics, and Environmental Sciences, University of Texas Health Science Center, Houston, TX, USA. Electronic address: eric.l.brown@uth.tmc.edu.

Funding

Genome-wide association to Staphylococcus carriageR01AI085014 · NIAID · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI BROWN, ERIC L, HANIS, CRAIG L · 2010 to 2013
$2.3M
NIAID NIH HHS R01 AI085014
6 · The paper itself

Abstract

Defects in innate immunity affect many different physiologic systems and several studies of patients with primary immunodeficiency disorders demonstrated the importance of innate immune system components in disease prevention or colonization of bacterial pathogens. To assess the role of the innate immune system on nasal colonization with Staphylococcus aureus, innate immune responses in pediatric S. aureus nasal persistent carriers (n = 14) and non-carriers (n = 15) were profiled by analyzing co-clustered gene sets (modules). We stimulated previously frozen peripheral blood mononuclear cells (PBMCs) from these subjects with i) a panel of TLR ligands, ii) live S. aureus (either a mixture of strains or stimulation with respective carriage isolates), or iii) heat-killed S. aureus. We found no difference in responses between carriers and non-carriers when PBMCs were stimulated with a panel of TLR ligands. However, PBMC gene expression profiles differed between persistent and non-S. aureus carriers following stimulation with either live or dead S. aureus. These observations suggest that individuals susceptible to persistent carriage with S. aureus may possess differences in their live/dead bacteria recognition pathway and that innate pathway signaling is different between persistent and non-carriers of S. aureus.

Indexed as

Carrier StateChildFemaleHumansImmunity, InnateLeukocytes, MononuclearMaleNasal MucosaStaphylococcal InfectionsStaphylococcus aureusTranscriptomeGene expressionNasal carriageRNAseqStaphylococcus aureusTargeted assayVita-PAMPs

Identifiers

PMID32758644
PMCPMC7722038

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.