Evidence map›Paper›PMID 32765272›Full record

ArticleFrontiers in pharmacology2020

Effect of Xiaoyaosan on Colon Morphology and Intestinal Permeability in Rats With Chronic Unpredictable Mild Stress.

Fengmin Ding, Jiajia Wu, Chenyue Liu, Qinglai Bian, Wenqi Qiu, Qingyu Ma, Xiaojuan Li, Man Long, Xiaojuan Zou, Jiaxu Chen

Open access · goldAbstract read
In one paragraph

Article in Frontiers in pharmacology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed, 1 pooled it
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 1 synthesis or guideline pooled it, 74 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Fengmin DingSchool of Basic Medical Science, Hubei University of Chinese Medicine, Wuhan, China.
Jiajia WuSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Chenyue LiuSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Qinglai BianSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Wenqi QiuSchool of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Qingyu MaFormula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou, China.
Xiaojuan LiFormula-pattern Research Center, School of Traditional Chinese Medicine, Jinan University, Guangzhou, China.
Man LongSchool of Basic Medical Science, Hubei University of Chinese Medicine, Wuhan, China.
Xiaojuan ZouSchool of Basic Medical Science, Hubei University of Chinese Medicine, Wuhan, China.
Jiaxu ChenSchool of Basic Medical Science, Hubei University of Chinese Medicine, Wuhan, China.
Beijing University of Chinese Medicine · CNHubei University of Chinese Medicine · CNJinan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIn our present study, a rat depression model induced by 6 weeks of chronic unpredictable mild stress (CUMS) was established, and we investigated how Xiaoyaosan affects the intestinal permeability of depressed rats and alterations in tight-junction proteins (TJs) involved in this process.

methodsThe rat depression model was established using CUMS for 6 consecutive weeks. A total of 40 healthy male Sprague-Dawley rats were randomly sorted into four groups: the control group, CUMS group, Xiaoyaosan group, and fluoxetine group. All groups, excluding the control group, were subjected to the 6-week CUMS program to generate the depression model. Body weight, food intake, and behaviors were observed during the modeling period. Histopathological alterations of colon tissue were evaluated by hematoxylin-eosin staining (H&E), and mucus-containing goblet cells were detected by periodic acid-Schiff (PAS) staining. The ultrastructural morphology of colonic mucosa was observed by transmission electron microscopy. Furthermore, immunohistochemistry (IHC) and quantitative reverse transcription polymerase chain reaction (qRT-PCR) were used to determine the expression of TJs. The concentrations of 5-hydroxytryptamine (5-HT) in the hypothalamus and colon were also assessed using enzyme-linked immunosorbent assay (ELISA).

resultsTreatment of depressed rats with Xiaoyaosan alleviated depression-like behaviors as demonstrated by increases in the total distance traveled, the number of entries into the central area in the open field test, the duration spent in the central area, and sucrose preference. Xiaoyaosan treatment also increased body weight gain and food intake in depressed rats. Moreover, Xiaoyaosan treatment effectively improved the colonic pathological and ultrastructural changes, upregulated the expression of ZO-1, occludin, and claudin-1 in the colon, and increased 5-HT levels in the hypothalamus and colonic mucosa.

conclusionsXiaoyaosan treatment attenuates depression-like behaviors caused by CUMS and ameliorates CUMS-induced abnormal intestinal permeability, which may be associated with the expression of TJs. These results suggest that Xiaoyaosan exerts an antidepressant effect that may be related to an improvement of intestinal barrier function

Indexed as

5-HTchronic unpredictable mild stressintestinal permeabilitytight junction proteinXiaoyaosan

Identifiers

PMID32765272
PMCPMC7378849
OpenAlexW3043023031

What Socratic holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.