Evidence map›Paper›PMID 32790754›Full record

ArticlePloS one2020

Integrated analysis of DNA methylation profile of HLA-G gene and imaging in coronary heart disease: Pilot study.

Concetta Schiano, Giuditta Benincasa, Teresa Infante, Monica Franzese, Rossana Castaldo, Carmela Fiorito, Gelsomina Mansueto, Vincenzo Grimaldi, Giovanni Della Valle, Gerardo Fatone and 6 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 37 citations in OpenAlex.

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  8. Epigenetics · 2022
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  17. DNA methylation profiling of CD04Annals of medicine and surgery (2012) · 2020
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 5 institutions in 1 country.

Concetta SchianoDepartment of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "L. Vanvitelli", Naples, Italy.ORCID 0000-0002-2570-6910
Giuditta BenincasaDepartment of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "L. Vanvitelli", Naples, Italy.
Teresa InfanteDepartment of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "L. Vanvitelli", Naples, Italy.
Monica FranzeseIRCCSSDN, Naples, Italy.ORCID 0000-0002-6490-7694
Rossana CastaldoIRCCSSDN, Naples, Italy.
Carmela FioritoU.O.C. Division of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], University of Campania "L. Vanvitelli", Naples, Italy.
Gelsomina MansuetoDepartment of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "L. Vanvitelli", Naples, Italy.
Vincenzo GrimaldiDivision of Clinical Immunology, Immunohematology, Transfusion Medicine and Transplant Immunology [SIMT], Clinical Department of Internal Medicine and Specialistic Units, Regional Reference Laboratory of Transplant Immunology [LIT], Azienda Universitaria Policlinico (AOU), Naples, Italy.
Giovanni Della ValleDepartment of Veterinary Medicine and Animal Production, University of Napoli Federico II, Napoli, Italy.
Gerardo FatoneDepartment of Veterinary Medicine and Animal Production, University of Napoli Federico II, Napoli, Italy.
Andrea SoricelliIRCCSSDN, Naples, Italy.
Giovanni Francesco NicolettiMultidisciplinary Department of Medical, Surgical and Dental Sciences, Plastic Surgery Unit, University of Campania "L. Vanvitelli", Naples, Italy.
Antonio RuoccoCardiovascular Diseases Department, "Cardarelli Hospital", Naples, Italy.
Ciro MauroCardiovascular Diseases Department, "Cardarelli Hospital", Naples, Italy.
Marco SalvatoreIRCCSSDN, Naples, Italy.
Claudio NapoliDepartment of Advanced Medical and Surgical Sciences (DAMSS), University of Campania "L. Vanvitelli", Naples, Italy.
University of Campania "Luigi Vanvitelli" · ITSDN Istituto di Ricerca Diagnostica e Nucleare · ITFatebenefratelli Hospital · ITUniversity of Naples Federico II · ITParthenope University of Naples · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimsImmune endothelial inflammation, underlying coronary heart disease (CHD) related phenotypes, could provide new insight into the pathobiology of the disease. We investigated DNA methylation level of the unique CpG island of HLA-G gene in CHD patients and evaluated the correlation with cardiac computed tomography angiography (CCTA) features.

methodsThirty-two patients that underwent CCTA for suspected CHD were enrolled for this study. Obstructive CHD group included fourteen patients, in which there was a stenosis greater than or equal to 50% in one or more of the major coronary arteries detected; whereas subjects with Calcium (Ca) Score = 0, uninjured coronaries and with no obstructive CHD (no critical stenosis, NCS) were considered as control subjects (n = 18). For both groups, DNA methylation profile of the whole 5'UTR-CpG island of HLA-G was measured. The plasma soluble HLA-G (sHLA-G) levels were detected in all subjects by specific ELISA assay. Statistical analysis was performed using R software.

resultsFor the first time, our study reported that 1) a significant hypomethylation characterized three specific fragments (B, C and F) of the 5'UTR-CpG island (p = 0.05) of HLA-G gene in CHD patients compared to control group; 2) the hypomethylation level of one specific fragment of 161bp (+616/+777) positively correlated with coronary Ca score, a relevant parameter of CCTA (p<0.05) between two groups evaluated and was predictive for disease severity.

conclusionsReduced levels of circulating HLA-G molecules could derive from epigenetic marks. Epigenetics phenomena induce hypomethylation of specific regions into 5'UTR-CpG island of HLA-G gene in CHD patients with obstructive non critical stenosis vs coronary stenosis individuals.

Indexed as

DNA Methylation5' Untranslated RegionsAdultAgedCalciumCase-Control StudiesComputed Tomography AngiographyCoronary DiseaseCoronary StenosisCoronary VesselsCpG IslandsEpigenesis, GeneticFemaleHLA-G AntigensHumansMale5' Untranslated RegionsCalciumHLA-G Antigens

Identifiers

PMID32790754
PMCPMC7425923
OpenAlexW3049038765

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.