ArticleMolecular systems biology2020
Exploring the virulence gene interactome with CRISPR/dCas9 in the human malaria parasite.
Article in Molecular systems biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 59 citations in OpenAlex.
- A critical role of the histone lysine demethylase JmjC1 in regulating severe malaria-associated virulence genes in Plasmodium falciparum.Nature communications · 2026Article
- A metabolism-chromatin axis promotes differential ribosomal RNA transcription in the human malaria parasite.Nature communications · 2025Article
- Circadian Biology in Parasites: Beyond Known Mechanisms.Annual review of microbiology · 2025Review
- High-resolution map of the Plasmodium falciparum genome reveals MORC/ApiAP2-mediated links between distant, functionally related genes.Nature microbiology · 2025Article
- An ISWI-related chromatin remodeller regulates stage-specific gene expression in Toxoplasma gondii.Nature microbiology · 2025Article
- Ectopic overexpression ofMicrobiology spectrum · 2025Article
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- ThemSphere · 2024Article
- Unraveling the complexities of ApiAP2 regulation in Plasmodium falciparum.Trends in parasitology · 2024Review
- AeLife · 2024Article
- Article
- The genetic landscape of origins of replication in P. falciparum.Nucleic acids research · 2024Article
- The multifaceted roles of Myb domain-containing proteins in apicomplexan parasites.Current opinion in microbiology · 2023Review
- Decoding the impact of nuclear organization on antigenic variation in parasites.Nature microbiology · 2023Review
- The mitochondrion ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Discovery of RUF6 ncRNA-interacting proteins involved inLife science alliance · 2023Article
- A nuclear redox sensor modulates gene activation andProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Preclinical characterization and target validation of the antimalarial pantothenamide MMV693183.Nature communications · 2022Article
- Chemogenomics identifies acetyl-coenzyme A synthetase as a target for malaria treatment and prevention.Cell chemical biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Mutually exclusive expression of the var multigene family is key to immune evasion and pathogenesis in Plasmodium falciparum, but few factors have been shown to play a direct role. We adapted a CRISPR-based proteomics approach to identify novel factors associated with var genes in their natural chromatin context. Catalytically inactive Cas9 ("dCas9") was targeted to var gene regulatory elements, immunoprecipitated, and analyzed with mass spectrometry. Known and novel factors were enriched including structural proteins, DNA helicases, and chromatin remodelers. Functional characterization of PfISWI, an evolutionarily divergent putative chromatin remodeler enriched at the var gene promoter, revealed a role in transcriptional activation. Proteomics of PfISWI identified several proteins enriched at the var gene promoter such as acetyl-CoA synthetase, a putative MORC protein, and an ApiAP2 transcription factor. These findings validate the CRISPR/dCas9 proteomics method and define a new var gene-associated chromatin complex. This study establishes a tool for targeted chromatin purification of unaltered genomic loci and identifies novel chromatin-associated factors potentially involved in transcriptional control and/or chromatin organization of virulence genes in the human malaria parasite.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.