Evidence mapPaperPMID 32900843Full record

ArticleJournal of the American Society of Nephrology : JASN2020

Extracellular Matrix Injury of Kidney Allografts in Antibody-Mediated Rejection: A Proteomics Study.

Sergi Clotet-Freixas, Caitriona M McEvoy, Ihor Batruch, Chiara Pastrello, Max Kotlyar, Julie Anh Dung Van, Madhurangi Arambewela, Alex Boshart, Sofia Farkona, Yun Niu and 16 more

Open access · bronzeAbstract read
In one paragraph

Article in Journal of the American Society of Nephrology : JASN, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
5.7field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 44 citations in OpenAlex.

  1. Article
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  10. Review
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  13. Advances in proteomic profiling of pediatric kidney diseases.Pediatric nephrology (Berlin, Germany) · 2022
    Review
  14. Review
  15. Article
  16. Hyperosmolality in CHO cell culture: effects on the proteome.Applied microbiology and biotechnology · 2022
    Article
  17. Article
  18. Article
  19. Pathway Enrichment Analysis of Microarray Data.Methods in molecular biology (Clifton, N.J.) · 2022
    Article
  20. Clinical Significances of Anti-Collagen Type I and Type III Antibodies in Antibody-Mediated Rejection.Transplant international : official journal of the European Society for Organ Transplantation · 2022
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 4 institutions in 3 countries.

Sergi Clotet-FreixasToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada Sergi.ClotetFreixas@uhnresearch.ca Ana.Konvalinka@uhn.ca.ORCID 0000-0002-6965-4403
Caitriona M McEvoyToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.ORCID 0000-0002-3179-5542
Ihor BatruchDepartment of Laboratory Medicine and Pathobiology, Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada.
Chiara PastrelloKrembil Research Institute, University Health Network, Toronto, Ontario, Canada.
Max KotlyarKrembil Research Institute, University Health Network, Toronto, Ontario, Canada.
Julie Anh Dung VanToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.ORCID 0000-0003-2419-7736
Madhurangi ArambewelaToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Alex BoshartToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Sofia FarkonaToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Yun NiuKrembil Research Institute, University Health Network, Toronto, Ontario, Canada.
Yanhong LiToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada Sergi.ClotetFreixas@uhnresearch.ca Ana.Konvalinka@uhn.ca.
Olusegun FamureToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Andrea BozovicDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Vathany KulasingamDepartment of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
Peixuen ChenToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
S Joseph KimToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Emilie ChanDivision of Nephrology, Department of Medicine, University Health Network, Toronto, Ontario, Canada.
Sajad MoshkelgoshaToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Syed Ashiqur RahmanCenter for Systems Immunology, Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.
Jishnu DasCenter for Systems Immunology, Department of Immunology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania.ORCID 0000-0002-5747-064X
Tereza MartinuToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Stephen JuvetToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Igor JurisicaKrembil Research Institute, University Health Network, Toronto, Ontario, Canada.ORCID 0000-0002-2507-946X
Andrzej ChruscinskiToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Rohan JohnToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.
Ana KonvalinkaToronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada Sergi.ClotetFreixas@uhnresearch.ca Ana.Konvalinka@uhn.ca.ORCID 0000-0001-6672-355X
University Health Network · CAUniversity of Pittsburgh · USUniversity of Toronto · CAMount Sinai Hospital · CA

Funding

CIHR
6 · The paper itself

Abstract

backgroundAntibody-mediated rejection (AMR) accounts for >50% of kidney allograft loss. Donor-specific antibodies (DSA) against HLA and non-HLA antigens in the glomeruli and the tubulointerstitium cause AMR while inflammatory cytokines such as TNF

methodsUnbiased proteomic analysis of laser-captured and microdissected glomeruli and tubulointerstitium was performed on 30 for-cause kidney biopsy specimens with early AMR, acute cellular rejection (ACR), or acute tubular necrosis (ATN).

resultsA total of 107 of 2026 glomerular and 112 of 2399 tubulointerstitial proteins was significantly differentially expressed in AMR versus ACR; 112 of 2026 glomerular and 181 of 2399 tubulointerstitial proteins were significantly dysregulated in AMR versus ATN (

conclusionsBasement membranes are often remodeled in chronic AMR. Proteomic analysis performed on laser-captured and microdissected glomeruli and tubulointerstitium identified early ECM remodeling, which may represent a new therapeutic opportunity.

Indexed as

AdultAgedAllograftsAntibodiesBasement MembraneBiopsyCathepsinsCell LineCysteine EndopeptidasesExtracellular MatrixFemaleGalectin 1Gene ExpressionGlutathione TransferaseGraft RejectionHistocompatibility Antigens Class IAntibodiesCathepsinsCTSV protein, humanCysteine EndopeptidasesGalectin 1Glutathione TransferaseGSTO1 protein, humanHistocompatibility Antigens Class ILamininlaminin gamma 1LGALS1 protein, humanMatrix Metalloproteinase 2Matrix Metalloproteinase 3Membrane ProteinsMMP2 protein, humanMMP3 protein, humannephrinPTPRO protein, humanReceptor-Like Protein Tyrosine Phosphatases, Class 3Tumor Necrosis Factor-alphaacute allograft rejectionextracellular matrixglomerular endothelial cellskidney transplantationproximal tubulerenal biopsy

Identifiers

PMID32900843
PMCPMC7608967
OpenAlexW3084307842

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.