ArticleProceedings of the National Academy of Sciences of the United States of America2020
Tuning the Baird aromatic triplet-state energy of cyclooctatetraene to maximize the self-healing mechanism in organic fluorophores.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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32 citing papers in PubMed, 66 citations in OpenAlex.
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- Singlet machine learning photodynamics reveal competing inversion paths of methylated cyclooctatetrathiophene.Chemical science · 2026Article
- Single-Molecule Fluorescence Defines a New Dye Chemistry.Chemical & biomedical imaging · 2026Review
- Experimental Study Redefines the Mechanism of Heptamethine Cyanine Phototruncation.Journal of the American Chemical Society · 2026Article
- Local GPCR density tips the balance of μ-opioid receptor trafficking.bioRxiv : the preprint server for biology · 2026Article
- Parallel stopped-flow interrogation of diverse biological systems at the single-molecule scale.Nature methods · 2026Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Modular Design and Scaffold-Synthesis of Multi-Functional Fluorophores for Targeted Cellular Imaging and Pyroptosis.Angewandte Chemie (International ed. in English) · 2025Article
- Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Concealed antiaromaticity.Open research Europe · 2025Article
- Counterion Exchange Enhances the Brightness and Photostability of a Fluorous Cyanine Dye.Chemistry (Weinheim an der Bergstrasse, Germany) · 2024Article
- Single-Molecule Imaging of Integral Membrane Protein Dynamics and Function.Annual review of biophysics · 2024Review
- Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation.Nature methods · 2024Article
- Enzyme-activatable charge transfer in gold nanoclusters.Chemical science · 2024Article
- Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024Review
- The potential of fluorogenicity for single molecule FRET and DyeCycling.QRB discovery · 2024Review
- Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Leveraging Baird aromaticity for advancement of bioimaging applications.Journal of physical organic chemistry · 2023Article
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Authors and funding
17 authors at 5 institutions in 2 countries.
Funding
Abstract
Bright, photostable, and nontoxic fluorescent contrast agents are critical for biological imaging. "Self-healing" dyes, in which triplet states are intramolecularly quenched, enable fluorescence imaging by increasing fluorophore brightness and longevity, while simultaneously reducing the generation of reactive oxygen species that promote phototoxicity. Here, we systematically examine the self-healing mechanism in cyanine-class organic fluorophores spanning the visible spectrum. We show that the Baird aromatic triplet-state energy of cyclooctatetraene can be physically altered to achieve order of magnitude enhancements in fluorophore brightness and signal-to-noise ratio in both the presence and absence of oxygen. We leverage these advances to achieve direct measurements of large-scale conformational dynamics within single molecules at submillisecond resolution using wide-field illumination and camera-based detection methods. These findings demonstrate the capacity to image functionally relevant conformational processes in biological systems in the kilohertz regime at physiological oxygen concentrations and shed important light on the multivariate parameters critical to self-healing organic fluorophore design.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.