Evidence map›Paper›PMID 32913205›Full record

ArticleScientific reports2020

Transcriptional analysis of cleft palate in TGFβ3 mutant mice.

J Liu, S K Chanumolu, K M White, M Albahrani, H H Otu, A Nawshad

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. mEpigenetics · 2022
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

J Liu *Department of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Lincoln, NE, 68583, USA.
S K Chanumolu *Department of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
K M WhiteDepartment of Growth and Development, College of Dentistry, University of Nebraska Medical Center College of Dentistry, Lincoln, NE, 68583, USA.
M AlbahraniDepartment of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
H H OtuDepartment of Electrical and Computer Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68588, USA.
A NawshadDepartment of Oral Biology, College of Dentistry, University of Nebraska Medical Center, Lincoln, NE, 68583, USA. anawshad@unmc.edu.
University of Nebraska–Lincoln · USUniversity of Nebraska Medical Center · US

Funding

TGFbeta3 SIGNALING IN DEVELOPMENT OF PALATER01DE017986 · NIDCR · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI NAWSHAD, ALI · 2008 to 2012
$1.8M
NIDCR NIH HHS R01 DE017986NIH HHS R01DE017986
6 · The paper itself

Abstract

Cleft palate (CP) is one of the most common craniofacial birth defects, impacting about 1 in 800 births in the USA. Tgf-β3 plays a critical role in regulating murine palate development, and Tgf-β3 null mutants develop cleft palate with 100% penetrance. In this study, we compared global palatal transcriptomes of wild type (WT) and Tgf-β3 -/- homozygous (HM) mouse embryos at the crucial palatogenesis stages of E14.5, and E16.5, using RNA-seq data. We found 1,809 and 2,127 differentially expressed genes at E16.5 vs. E14.5 in the WT and HM groups, respectively (adjusted p < 0.05; |fold change|> 2.0). We focused on the genes that were uniquely up/downregulated in WT or HM at E16.5 vs. E14.5 to identify genes associated with CP. Systems biology analysis relating to cell behaviors and function of WT and HM specific genes identified functional non-Smad pathways and preference of apoptosis to epithelial-mesenchymal transition. We identified 24 HM specific and 11 WT specific genes that are CP-related and/or involved in Tgf-β3 signaling. We validated the expression of 29 of the 35 genes using qRT-PCR and the trend of mRNA expression is similar to that of RNA-seq data . Our results enrich our understanding of genes associated with CP that are directly or indirectly regulated via TGF-β.

Indexed as

Gene Expression RegulationMutationTranscriptomeAnimalsCleft PalateEmbryo, MammalianEpithelial-Mesenchymal TransitionFemaleMaleMiceMice, Inbred C57BLMice, KnockoutRNA-SeqSignal TransductionTransforming Growth Factor beta3Tgfb3 protein, mouseTransforming Growth Factor beta3

Identifiers

PMID32913205
PMCPMC7483747
OpenAlexW3083781677

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.