Evidence map›Paper›PMID 32914385›Full record

ArticleIn vitro cellular & developmental biology. Animal2020

Discriminating aspects of global metabolism of neonatal cardiomyocytes from wild type and KO-CSRP3 rats using proton magnetic resonance spectroscopy of culture media samples.

Antonio Carlos Bloise, Jennifer Adriane Dos Santos, Isis Vasconcelos de Brito, Vinicius Bassaneze, Ligia Ferreira Gomes, Adriano Mesquita Alencar

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 6 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Antonio Carlos BloiseLaboratory of Microrheology and Molecular physiology, Instituto de Física, Universidade de São Paulo, São Paulo, 05508-090, Brazil. acbloise@if.usp.br.ORCID https://orcid.org/0000-0002-8529-1577
Jennifer Adriane Dos SantosLaboratory of Microrheology and Molecular physiology, Instituto de Física, Universidade de São Paulo, São Paulo, 05508-090, Brazil.
Isis Vasconcelos de BritoLaboratory of Microrheology and Molecular physiology, Instituto de Física, Universidade de São Paulo, São Paulo, 05508-090, Brazil.
Vinicius BassanezeLaboratory of Genetics and Molecular Cardiology/LIM 13, Heart Institute (InCor), University of São Paulo Medical School, São Paulo, 05403-000, Brazil.
Ligia Ferreira GomesLaboratory of Microrheology and Molecular physiology, Instituto de Física, Universidade de São Paulo, São Paulo, 05508-090, Brazil.
Adriano Mesquita AlencarLaboratory of Microrheology and Molecular physiology, Instituto de Física, Universidade de São Paulo, São Paulo, 05508-090, Brazil.
Universidade de São Paulo · BR

Funding

Brazilian Center for Research in Energy and Materials-Brasilizan Biosciences National Laboratory 20992Fundação de Amparo à Pesquisa do Estado de São Paulo 2011/19678-1Fundação de Amparo à Pesquisa do Estado de São Paulo 2013/17368-0Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/21646-9Fundação de Amparo à Pesquisa do Estado de São Paulo 2014/22102-2Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/20910-5
6 · The paper itself

Abstract

Knockout of multifunction gene cysteine- and glycine-rich protein 3 (CSRP3) in cardiomyocytes (CMs) of mice leads to heart dilation, severely affecting its functions. In humans, CSRP3 mutations are associated with hypertrophic (HCM) and dilated cardiomyopathy (DCM). The absence of the CSRP3 expression produces unknown effects on in vitro neonatal CMs' metabolism. The metabolome changes in culture media conditioned by CSRP3 knockout (KO-CSRP3), and wild type (WT) neonatal cardiomyocytes were investigated under untreated or after metabolic challenging conditions produced by isoproterenol (ISO) stimulation, by in vitro high-resolution proton magnetic resonance spectroscopy (

Indexed as

Proton Magnetic Resonance SpectroscopyAnimalsAnimals, NewbornCell ShapeCulture MediaDiscriminant AnalysisIsoproterenolLeast-Squares AnalysisLIM Domain ProteinsMuscle ProteinsMyocytes, CardiacRatsStatistics as TopicCulture Mediacysteine and glycine-rich protein 3IsoproterenolLIM Domain ProteinsMuscle ProteinsCardiomyocytesCysteine- and glycine-rich protein 3Knockout animalMetabolomicsProton magnetic resonance spectroscopy

Identifiers

PMID32914385
OpenAlexW3085394665

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.