Evidence map›Paper›PMID 32933205›Full record

ArticleCells2020

An Evolutionary Cancer Epigenetic Approach Revealed DNA Hypermethylation of Ultra-Conserved Non-Coding Elements in Squamous Cell Carcinoma of Different Mammalian Species.

Luca Morandi, Silvia Sabattini, Andrea Renzi, Antonella Rigillo, Giuliano Bettini, Eva Dervas, Alexandria Schauer, Marco Morandi, Davide B Gissi, Achille Tarsitano and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Luca MorandiFunctional MR Unit, Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy.ORCID 0000-0002-3810-9760
Silvia SabattiniDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano Emilia, Italy.ORCID 0000-0001-7005-2736
Andrea RenziDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano Emilia, Italy.ORCID 0000-0003-4004-8407
Antonella RigilloDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano Emilia, Italy.
Giuliano BettiniDepartment of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano Emilia, Italy.
Eva DervasInstitute of Veterinary Pathology, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
Alexandria SchauerInstitute of Veterinary Pathology, Vetsuisse Faculty, University of Bern, 8466 Bern, Switzerland.
Marco MorandiFunctional MR Unit, Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy.
Davide B GissiSection of Oral Science, Department of Biomedical and Neuromuscular Sciences, University of Bologna, 40125 Bologna, Italy.ORCID 0000-0002-0195-7694
Achille TarsitanoUnit of Oral and Maxillofacial Surgery, Azienda Ospedaliero-Universitaria di Bologna, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40138 Bologna, Italy.ORCID 0000-0002-5815-7591
Stefania EvangelistiFunctional MR Unit, Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy.
Caterina TononFunctional MR Unit, Bellaria Hospital, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40139 Bologna, Italy.
University of Bologna · ITAzienda USL di Bologna · ITIstituto delle Scienze Neurologiche di Bologna · ITUniversity of Bern · CHUniversity of Zurich · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundUltra-conserved non-coding elements (UCNEs) are genomic sequences that exhibit > 95% sequence identity between humans, mammals, birds, reptiles, and fish. Recent findings reported their functional role in cancer. The aim of this study was to evaluate the DNA methylation modifications of UNCEs in squamous cell carcinoma (SCC) from different mammal species.

methodsFifty SCCs from 26 humans, 17 cats, 3 dogs, 1 horse, 1 bovine, 1 badger, and 1 porcupine were investigated. Fourteen feline stomatitis and normal samples from 36 healthy human donors, 7 cats, 5 dogs, 5 horses, 2 bovines and 1 badger were collected as normal controls. Bisulfite next generation sequencing evaluated the DNA methylation level from seven UCNEs (uc.160, uc.283, uc.416, uc.339, uc.270, uc.299, and uc.328).

results57/59 CpGs were significantly different according to the Kruskal-Wallis test (

conclusionsOur findings indicate that UCNEs are hypermethylated in human SCC, and this behavior is also conserved among different species of mammals.

Indexed as

Epigenesis, GeneticEvolution, MolecularAgedAnimalsCarcinoma, Squamous CellCatsCattleConserved SequenceCpG IslandsDNA MethylationDogsFemaleHorsesHumansMaleMouth Neoplasmsbisulfite sequencingDNA methylationevolutionary epigeneticssquamous cell carcinomaultra-conserved non-coding elements

Identifiers

PMID32933205
PMCPMC7565279
OpenAlexW3084454476

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.