ArticleScientific reports2020
A high throughput screening system for studying the effects of applied mechanical forces on reprogramming factor expression.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 11 citations in OpenAlex.
- A Computational Model of Mechanical Stretching of Cultured Cells on a Flexible Membrane.Research square · 2026Article
- Potential use of human pluripotency-related gene expression reporter cell line for screening small molecules to enhance induction of pluripotency.BMB reports · 2025Article
- A high throughput cell stretch device for investigating mechanobiologyAPL bioengineering · 2024Article
- A high throughput blood-brain barrier model incorporating shear stress with improved predictive power for drug discovery.Biomicrofluidics · 2023Article
- The effects of strain history on aortic valve interstitial cell activation in a 3D hydrogel environment.APL bioengineering · 2023Article
- Biomechanical regulation of breast cancer metastasis and progression.Scientific reports · 2021Article
- Mechanobiological conditioning of mesenchymal stem cells for enhanced vascular regeneration.Nature biomedical engineering · 2021Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical forces are important in the regulation of physiological homeostasis and the development of disease. The application of mechanical forces to cultured cells is often performed using specialized systems that lack the flexibility and throughput of other biological techniques. In this study, we developed a high throughput platform for applying complex dynamic mechanical forces to cultured cells. We validated the system for its ability to accurately apply parallel mechanical stretch in a 96 well plate format in 576 well simultaneously. Using this system, we screened for optimized conditions to stimulate increases in Oct-4 and other transcription factor expression in mouse fibroblasts. Using high throughput mechanobiological screening assays, we identified small molecules that can synergistically enhance the increase in reprograming-related gene expression in mouse fibroblasts when combined with mechanical loading. Taken together, our findings demonstrate a new powerful tool for investigating the mechanobiological mechanisms of disease and performing drug screening in the presence of applied mechanical load.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.