Evidence map›Paper›PMID 32995700›Full record

ArticlePlant direct2020

Live-cell RESOLFT nanoscopy of transgenic

Sebastian Schnorrenberg, Hassan Ghareeb, Lars Frahm, Tim Grotjohann, Nickels Jensen, Thomas Teichmann, Stefan W Hell, Volker Lipka, Stefan Jakobs

Open access · goldAbstract read
In one paragraph

Article in Plant direct, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
8.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 42 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sebastian SchnorrenbergDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.ORCID https://orcid.org/0000-0002-8076-2237
Hassan GhareebDepartment of Plant Cell Biology Albrecht-von-Haller Institute of Plant Sciences University of Göttingen Göttingen Germany.
Lars FrahmDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.
Tim GrotjohannDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.
Nickels JensenDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.ORCID https://orcid.org/0000-0002-0085-8942
Thomas TeichmannDepartment of Plant Cell Biology Albrecht-von-Haller Institute of Plant Sciences University of Göttingen Göttingen Germany.
Stefan W HellDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.ORCID https://orcid.org/0000-0002-9638-5077
Volker LipkaDepartment of Plant Cell Biology Albrecht-von-Haller Institute of Plant Sciences University of Göttingen Göttingen Germany.
Stefan JakobsDepartment of NanoBiophotonics Max Planck Institute for Biophysical Chemistry Göttingen Germany.ORCID https://orcid.org/0000-0002-8028-3121
Max Planck Institute for Biophysical Chemistry · DEUniversity of Göttingen · DEUniversitätsmedizin Göttingen · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Subdiffraction super-resolution fluorescence microscopy, or nanoscopy, has seen remarkable developments in the last two decades. Yet, for the visualization of plant cells, nanoscopy is still rarely used. In this study, we established RESOLFT nanoscopy on living green plant tissue. Live-cell RESOLFT nanoscopy requires and utilizes comparatively low light doses and intensities to overcome the diffraction barrier. We generated a transgenic

Indexed as

fluorescence lifetime gatinglive cell imagingplantrsEGFPsuper‐resolution microscopy

Identifiers

PMID32995700
PMCPMC7507094
OpenAlexW3083334395

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.