ArticlePLoS neglected tropical diseases2020
Comparison and clinical validation of qPCR assays targeting Leishmania 18S rDNA and HSP70 genes in patients with American Tegumentary Leishmaniasis.
Article in PLoS neglected tropical diseases, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 49 citations in OpenAlex.
- Exploring real-time PCR techniques for diagnosing leishmaniasis: key insights from a systematic review.Parasitology research · 2025Pooled it
- Evaluation of the Loop-Mediated Isothermal Amplification (LAMP) Technique in Swab Samples from Ulcerated Cutaneous Lesions Compared with Conventional Diagnostic Methods for American Tegumentary Leishmaniasis in Patients Treated at a Reference Center in Rio de Janeiro, Brazil.Tropical medicine and infectious disease · 2026Article
- Molecular Detection of Dengue and Malaria Parasites in Field-Collected Mosquitoes from Meta, Colombia: Implications for Vector-Borne Disease Surveillance.Epidemiologia (Basel, Switzerland) · 2026Article
- Integrating molecular tools into leishmaniosis surveillance: evaluation of a commercial qPCR kit in sand fly vectors.Parasites & vectors · 2026Article
- Identification of Leishmania spp. and Trypanosoma cruzi in bats captured in El Paso County, Texas.PLoS neglected tropical diseases · 2026Article
- From Skin to Sequence: Pan-species Detection and Speciation of Leishmania.The Journal of infectious diseases · 2026Article
- Extracellular Vesicle Release from Immune Cells in Cutaneous Leishmaniasis: Modulation byPathogens (Basel, Switzerland) · 2025Article
- Validation of a Real-Time PCR for the Diagnosis ofTropical medicine and infectious disease · 2025Article
- High detection rate of parasitic load by qPCR targeting 18S rDNA in blood of patients with active leishmaniasis lesions.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Article
- The activity of miltefosine combined with oral probiotics in a mouse model of cutaneous leishmaniasis.Memorias do Instituto Oswaldo Cruz · 2025Article
- A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples.Parasite (Paris, France) · 2025Article
- Standardization and Evaluation of the LAMP Technique for the Diagnosis of Canine Visceral Leishmaniasis in Conjunctival Swab Samples Using DNA Extracted by a Silica Column and Boiling.Tropical medicine and infectious disease · 2024Article
- Descriptive and molecular epidemiology of leishmaniasis diagnosed from clinical samples in the United States, 2021-2022.Microbiology spectrum · 2024Article
- Abundance and Leishmania infection patterns of the sand fly Psathyromyia cratifer in Southern Mexico.PLoS neglected tropical diseases · 2024Article
- Comparative Study of Colorimetric In Situ Hybridization and Quantitative Real-Time Polymerase Chain Reaction for Diagnosis of Infection byTropical medicine and infectious disease · 2024Article
- Article
- Recombinase-based amplification coupled with lateral flow chromatography for the specific and sensitive detection and identification ofFrontiers in microbiology · 2024Article
- Validation of Oxford nanopore sequencing for improved New World Leishmania species identification via analysis of 70-kDA heat shock protein.Parasites & vectors · 2023Article
- Clinical performance of a quantitative pan-genusPractical laboratory medicine · 2023Article
- HRM Accuracy and Limitations as a Species Typing Tool forInternational journal of molecular sciences · 2023Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leishmaniasis is a worldwide neglected disease, encompassing asymptomatic infections and different clinical forms, such as American Tegumentary Leishmaniasis (ATL) which is part of the complex of diseases caused by protozoan parasites from Leishmania genus, transmitted by sand fly vectors. As a neglected disease, much effort is still needed in treatment and diagnosis. Currently, ATL diagnosis is mainly made by parasite detection by microscopy. The sensitivity of the method varies, and factors such as collection procedures interfere. Molecular approaches, specially based on Real Time PCR (qPCR) technique, has been widely used to detect Leishmania infection and to quantify parasite load, once it is a simple, rapid and sensitive methodology, capable to detect low parasite concentrations and less prone to variability. Although many studies have been already published addressing the use of this technique, an improvement on these methodologies, including an analytical validation, standardization and data association is demanded. Moreover, a proper validation by the assay by the use of clinical samples is still required. In this sense, the purpose of the present work is to compare the performance of qPCR using two commonly used targets (18S rDNA and HSP70) with an internal control (RNAse P) in multiplex reactions. Additionally, we validated reactions by assaying 88 samples from patients presenting different clinical forms of leishmaniasis (cutaneous, mucosal, recent and old lesions), representing the diversity found in Brazil's Amazon Region. Following the methodology proposed herein, the results indicate the use of both qPCR assays, 18S rDNA and HSP70, to achieve a very good net sensitivity (98.5%) and specificity (100%), performing simultaneous or sequential testing, respectively. With this approach, our main goal is to conclude the first step of a further multicenter study to propose the standardization of detection and quantification of Leishmania.
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