ArticleBMC genomics2020
Comparative transcriptome analysis reveals that PCK1 is a potential gene affecting IMF deposition in buffalo.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
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- Identification of novel biomarkers and therapeutic targets for type II ketosis in dairy cows through metabolomics and snRNA-Seq.Functional & integrative genomics · 2026Article
- RNA sequencing reveals lncRNAs that specifically regulate unsaturated fatty acid generation in buffaloes.Archives animal breeding · 2026Article
- Bta-novel-miR-25336 enhanced adipose differentiation and unsaturated fatty acid synthesis by targeting ACADS in intramuscular adipocytes of river buffalo.BMC genomics · 2025Article
- Identification of Key Genes Related to Intramuscular Fat Content of Psoas Major Muscle in Saba Pigs by Integrating Bioinformatics and Machine Learning Based on Transcriptome Data.Animals : an open access journal from MDPI · 2025Article
- Crossbreeding Simmental with Mongolian, and Holstein cattle can improve feed efficiency and energy metabolism by upregulating COX3 and downregulating PRSS2 gene expression.Frontiers in nutrition · 2025Article
- Identification of highly expressed genes and efficient core promoters specific to buffalo skeletal muscles.Archives animal breeding · 2025Article
- Current status of transcriptome sequencing technology in ruminants.Frontiers in veterinary science · 2025Review
- A review of emerging technologies, nutritional practices, and management strategies to improve intramuscular fat composition in beef cattle.Animal biotechnology · 2024Review
- Fat deposition and partitioning for meat production in cattle and sheep.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Review
- Isolation methods, proliferation, and adipogenic differentiation of adipose-derived stem cells from different fat depots in bovines.Molecular and cellular biochemistry · 2024Article
- miR-128-3p inhibits intramuscular adipocytes differentiation in chickens by downregulating FDPS.BMC genomics · 2023Article
- Effects of HOXC8 on the Proliferation and Differentiation of Porcine Preadipocytes.Animals : an open access journal from MDPI · 2023Article
- Integrated Comparative Transcriptome and circRNA-lncRNA-miRNA-mRNA ceRNA Regulatory Network Analyses Identify Molecular Mechanisms Associated with Intramuscular Fat Content in Beef Cattle.Animals : an open access journal from MDPI · 2023Article
- IMF deposition ceRNA network analysis and functional study of HIF1a in yak.Frontiers in veterinary science · 2023Article
- Comparative transcriptome analysis of longissimus dorsi tissues with different intramuscular fat contents from Guangling donkeys.BMC genomics · 2022Article
- Article
- Characterization of novelEvolutionary applications · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- FATP1 Exerts Variable Effects on Adipogenic Differentiation and Proliferation in Cells Derived From Muscle and Adipose Tissue.Frontiers in veterinary science · 2022Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundIn China, although buffaloes are abundant, beef is mainly obtained from cattle, and this preference is mainly attributed to the low intramuscular fat (IMF) content of buffalo. Genetic factors are an important driver that affects IMF deposition.
resultsTo reveal the intrinsic factors responsible for the low IMF content of buffalo, mRNA expression patterns in muscle and adipose tissue between buffalo and cattle were characterized by RNA sequencing analysis. The IMF content in Nanyang cattle was higher than that in Xinyang buffalo. A total of 1566 mRNAs expressed in adipose tissue showed differential expression between the longissimus dorsi muscles of buffalo and cattle. Functional annotation suggested a difference in the glycolysis/gluconeogenesis pathway between the two species. The results of RT-qPCR analysis and gain-of-function experiments confirmed the positive association between the IMF content and phosphoenolpyruvate carboxykinase 1 (PCK1) expression in buffalo. In both mouse C2C12 cells and cultured bovine myocytes, the activity of the PCK1 promoter in buffalo is lower than that in cattle. However, in mouse 3T3-L1 adipocytes and cultured bovine adipocytes, the activity of PCK1 in buffalo promoter is higher than that in cattle.
conclusionsThese results indicate the important role of PCK1 in buffalo IMF deposition and illustrate the differences between buffalo and cattle promoter activity that drive PCK1 expression. This research helps to establish a foundation for further studies investigating IMF deposition in buffalo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.