Evidence map›Paper›PMID 33047280›Full record

ArticleStem cell reviews and reports2020

Comparative Metabolomic Profiling of Rat Embryonic and Induced Pluripotent Stem Cells.

Vladimir V Sherstyuk, Lyudmila V Yanshole, Ekaterina A Zelentsova, Arsenty D Melnikov, Sergey P Medvedev, Yuri P Tsentalovich, Suren M Zakian

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in Stem cell reviews and reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Vladimir V SherstyukFederal Research Center Institute of Cytology and Genetics SB RAS, 10 Lavrentyeva Ave, Novosibirsk, 630090, Russia.
Lyudmila V YansholeFederal Research Center Institute of Cytology and Genetics SB RAS, 10 Lavrentyeva Ave, Novosibirsk, 630090, Russia.
Ekaterina A ZelentsovaInternational Tomography Center SB RAS, Institutskaya 3a, 630090, Novosibirsk, Russia.
Arsenty D MelnikovInternational Tomography Center SB RAS, Institutskaya 3a, 630090, Novosibirsk, Russia.
Sergey P MedvedevFederal Research Center Institute of Cytology and Genetics SB RAS, 10 Lavrentyeva Ave, Novosibirsk, 630090, Russia.
Yuri P TsentalovichInternational Tomography Center SB RAS, Institutskaya 3a, 630090, Novosibirsk, Russia.
Suren M ZakianFederal Research Center Institute of Cytology and Genetics SB RAS, 10 Lavrentyeva Ave, Novosibirsk, 630090, Russia. zakian@bionet.nsc.ru.ORCID 0000-0003-2448-6511

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Metabolomic profiles of somatic cells, embryonic stem cells (ESCs), and induced pluripotent stem cells (iPSCs) reflect their metabolic phenotypes. The comparative study of metabolomes of these cells is important for understanding the differences in metabolism between somatic and pluripotent cells, and also the possible differences between ESCs and iPSCs. Here, we performed for the first time the metabolomic analysis of rat ESCs, iPSCs, and embryonic fibroblasts (EFs) at both quantitative and semi-quantitative levels using NMR spectroscopy and liquid chromatography with mass spectrometric detection, respectively. The total of 106 metabolites has been identified, and the concentrations of 51 compounds have been measured. It is found that the reprogramming of rat EFs into iPSCs affects virtually all metabolic pathways and causes drastic changes in the cell metabolomic profile. The difference between ESCs and iPSCs is much less pronounced: the concentrations of the majority of metabolites in ESCs and iPSCs are similar, and significant differences were observed for only several compounds, including adenosine, cysteic acid, glycerophosphoglycerol, inositol phosphate, glucose, myo-inositol, phosphoserine, xanthosine, guanosine. The observed differences between the metabolomic compositions of ESCs and iPSCs do not influence the pluripotent ability of iPSCs. Graphical Abstract.

Indexed as

MetabolomicsAcetatesAnimalsEmbryonic Stem CellsInduced Pluripotent Stem CellsLactic AcidMagnetic Resonance SpectroscopyMetabolomePrincipal Component AnalysisRatsAcetatesLactic AcidEmbryonic stem cellsInduced pluripotent stem cellsLC-MSMetabolomicsNMRPluripotencyRatReprogramming

Identifiers

PMID33047280

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.