Evidence map›Paper›PMID 33073545›Full record

ReviewWIREs mechanisms of disease2021

Bioengineering tools for probing intracellular events in T lymphocytes.

Xinyuan Zhang, Chelsea F Mariano, Yuta Ando, Keyue Shen

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in WIREs mechanisms of disease, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. PANoptosis and Immune Remodeling in the Tumor Microenvironment.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Review
  2. Monitoring Dynamic of cAMP Signaling in Primary Murine CD4Methods in molecular biology (Clifton, N.J.) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Xinyuan ZhangDepartment of Biomedical Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-8401-7173
Chelsea F MarianoDepartment of Biomedical Engineering, University of Southern California, Los Angeles, California, USA.
Yuta AndoDepartment of Biomedical Engineering, University of Southern California, Los Angeles, California, USA.
Keyue ShenDepartment of Biomedical Engineering, University of Southern California, Los Angeles, California, USA.ORCID 0000-0001-9605-1635
University of Southern California · US

Funding

(PQ5) Microenvironmental Regulation of Mitochondrial Heterogeneity in Cancer MetastasisR01CA220012 · NCI · UNIVERSITY OF SOUTHERN CALIFORNIA · PI SHEN, KEYUE · 2019 to 2023
$2.2M
Artificial Stromal Cell for Hematopoietic Stem Cell ExpansionR21EB024748 · NIBIB · UNIVERSITY OF SOUTHERN CALIFORNIA · PI SHEN, KEYUE · 2017 to 2019
$637k
NCI NIH HHS R01 CA220012NIBIB NIH HHS R21 EB024748
6 · The paper itself

Abstract

T lymphocytes are the central coordinator and executor of many immune functions. The activation and function of T lymphocytes are mediated through the engagement of cell surface receptors and regulated by a myriad of intracellular signaling network. Bioengineering tools, including imaging modalities and fluorescent probes, have been developed and employed to elucidate the cellular events throughout the functional lifespan of T cells. A better understanding of these events can broaden our knowledge in the immune systems biology, as well as accelerate the development of effective diagnostics and immunotherapies. Here we review the commonly used and recently developed techniques and probes for monitoring T lymphocyte intracellular events, following the order of intracellular events in T cells from activation, signaling, metabolism to apoptosis. The techniques introduced here can be broadly applied to other immune cells and cell systems. This article is categorized under: Immune System Diseases > Molecular and Cellular Physiology Immune System Diseases > Biomedical Engineering Infectious Diseases > Biomedical Engineering.

Indexed as

Receptors, Antigen, T-CellT-LymphocytesBioengineeringBiomedical EngineeringSignal TransductionReceptors, Antigen, T-CellFRETImaging probesImmune cellsImmunological synapseLymphocytesT cell signaling

Identifiers

PMID33073545
PMCPMC8297511
OpenAlexW3093132960

What Socratic holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.