Evidence map›Paper›PMID 33086769›Full record

ArticleBiomedicines2020

CRP Is Transported by Monocytes and Monocyte-Derived Exosomes in the Blood of Patients with Coronary Artery Disease.

Ivan Melnikov, Sergey Kozlov, Olga Saburova, Ekaterina Zubkova, Olga Guseva, Sergey Domogatsky, Tatiana Arefieva, Natalia Radyukhina, Maria Zvereva, Yuliya Avtaeva and 2 more

Open access · goldAbstract read
In one paragraph

Article in Biomedicines, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Ivan MelnikovNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Sergey KozlovNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Olga SaburovaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Ekaterina ZubkovaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Olga GusevaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Sergey DomogatskyNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Tatiana ArefievaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Natalia RadyukhinaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Maria ZverevaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Yuliya AvtaevaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Lyudmila BuryachkovskayaNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
Zufar GabbasovNational Medical Research Centre of Cardiology of the Ministry of Health of the Russian Federation, 3-rd Cherepkovskaya Street, Moscow 121552 15A, Russia.
National Medical Research Center of Cardiology · RUInstitute of Biomedical Problems · RU

Funding

Russian Science Foundation #16-15-10098
6 · The paper itself

Abstract

The objective of this work was to study the ability of blood cells and their microparticles to transport monomeric and pentameric forms of C-reactive protein (mCRP and pCRP) in the blood of patients with coronary artery disease (CAD). Blood was obtained from 14 patients with CAD 46 ± 13 years old and 8 healthy volunteers 49 ± 13.6 years old. Blood cells and microparticles with mCRP and pCRP on their surface were detected by flow cytometry. Messenger RNA (mRNA) of CRP was extracted from peripheral blood monocytes stimulated with lipopolysaccharide (LPS) and granulocyte-macrophage colony-stimulating factor (GM-CSF). mRNA of CRP in monocytes was detected with PCR. Monocytes were predominantly pCRP-positive (92.9 ± 6.8%). mCRP was present on 22.0 ± 9.6% of monocyte-derived exosomes. mCRP-positive leukocyte-derived microparticle counts were significantly higher (8764 ± 2876/µL) in the blood of patients with CAD than in healthy volunteers (1472 ± 307/µL). LPS and GM-CSF stimulated monocytes expressed CRP mRNA transcripts levels (0.79 ± 0.73-fold), slightly lower relative to unstimulated hepatocytes of the HepG2 cell line (1.0 ± 0.6-fold), but still detectable. The ability of monocytes to transport pCRP in blood flow, and monocyte-derived exosomes to transmit mCRP, may contribute to the maintenance of chronic inflammation in CAD.

Indexed as

chronic inflammationcoronary artery diseaseexosomesmicroparticlesmonocytesmonomeric form of C-reactive proteinpentameric form of C-reactive protein

Identifiers

PMID33086769
PMCPMC7589628
OpenAlexW3093089933

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.