Evidence map›Paper›PMID 33100027›Full record

ArticleCartilage2021

DMOG Negatively Impacts Tissue Engineered Cartilage Development.

Jessica M Falcon, Dylan Chirman, Alyssa Veneziale, Justin Morman, Katherine Bolten, Shital Kandel, William Querido, Theresa Freeman, Nancy Pleshko

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cartilage, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 7 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jessica M FalconTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.ORCID 0000-0001-6829-1826
Dylan ChirmanTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Alyssa VenezialeTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Justin MormanTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Katherine BoltenTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Shital KandelTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
William QueridoTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Theresa FreemanDivision of Orthopaedic Research, Thomas Jefferson University, Philadelphia, PA, USA.
Nancy PleshkoTissue Imaging and Spectroscopy Lab, Department of Bioengineering, Temple University, Philadelphia, PA, USA.
Temple University · USThomas Jefferson University · US

Funding

Evaluation of Cartilage Tissue Engineering Strategies by IR ImagingR01AR056145 · NIAMS · TEMPLE UNIV OF THE COMMONWEALTH · PI PLESHKO, NANCY · 2009 to 2020
$3.3M
NIAMS NIH HHS R01 AR056145
6 · The paper itself

Abstract

objectiveArticular cartilage exists in a hypoxic environment, which motivates the use of hypoxia-simulating chemical agents to improve matrix production in cartilage tissue engineering. The aim of this study was to investigate whether dimethyloxalylglycine (DMOG), a HIF-1α stabilizer, would improve matrix production in 3-dimensional (3D) porcine synovial-derived mesenchymal stem cell (SYN-MSC) co-culture with chondrocytes.

designPellet cultures and scaffold-based engineered cartilage were grown

resultsThe addition of DMOG resulted in HIF-1α stabilization in the exterior of the engineered constructs, which resulted in increased regional type II collagen deposition, but the stabilization did not translate to overall increased extracellular matrix deposition. There was no increase in HIF-1α stabilization in the pellet cultures. DMOG treatment also negatively affected the mechanical competency of the engineered cartilage.

conclusionsDespite previous studies that demonstrated the efficacy of DMOG, here, short-term treatment with DMOG did not have a uniformly positive impact on the chondrogenic capacity of SYN-MSCs in either pellet culture or in scaffold-based engineered cartilage, as evidenced by reduced matrix production. Such 3D constructs generally have a naturally occurring hypoxic center, which allows for the stabilization of HIF-1α in the interior tissue. Thus, short-term addition of DMOG may not further improve this in cartilage tissue engineered constructs.

Indexed as

Cartilage, ArticularTissue EngineeringAmino Acids, DicarboxylicAnimalsChondrogenesisSwineAmino Acids, Dicarboxylicoxalylglycinecartilagecollagenmesenchymal stem cellstissue engineering

Identifiers

PMID33100027
PMCPMC8804718
OpenAlexW3093806267

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.