ArticleCell death and differentiation2021
Single-cell RNA-seq reveals novel mitochondria-related musculoskeletal cell populations during adult axolotl limb regeneration process.
Article in Cell death and differentiation, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Periosteal mitochondria DNA structures drive aging-associated poor skeletal repair.Bone research · 2026Article
- Current cutting-edge omics techniques on musculoskeletal tissues and diseases.Bone research · 2025Review
- Fibrocartilage hyalinization: A potential therapeutic strategy for articular fibrocartilage.Journal of orthopaedic translation · 2025Review
- Across the space: applications of spatial transcriptomic technology in healthy and diseased muscle.Frontiers in cell and developmental biology · 2025Review
- Mitochondrial dynamics govern whole-body regeneration through stem cell pluripotency and mitonuclear balance.Nature communications · 2024Article
- Unveiling axolotl transcriptome for tissue regeneration with high-resolution annotation via long-read sequencing.Computational and structural biotechnology journal · 2024Article
- Hyperplastic Human Macromass Cartilage for Joint Regeneration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Emerging roles of mitochondria in animal regeneration.Cell regeneration (London, England) · 2023Review
- Now that We Got There, What Next?Methods in molecular biology (Clifton, N.J.) · 2023Article
- The salamander blastema within the broader context of metazoan regeneration.Frontiers in cell and developmental biology · 2023Review
- Rebuilding limbs, one cell at a time.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Construction of the axolotl cell landscape using combinatorial hybridization sequencing at single-cell resolution.Nature communications · 2022Article
- Article
- The amazing and anomalous axolotls as scientific models.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Understanding the Transcriptomic Landscape to Drive New Innovations in Musculoskeletal Regenerative Medicine.Current osteoporosis reports · 2022Review
- Cell Hypertrophy: A "Biophysical Roadblock" to Reversing Kidney Injury.Frontiers in cell and developmental biology · 2022Article
- Muscles are barely required for the patterning and cell dynamics in axolotl limb regeneration.Frontiers in genetics · 2022Article
- Advances in single-cell sequencing and its application to musculoskeletal system research.Cell proliferation · 2022Review
- Large-scale integration of single-cell transcriptomic data captures transitional progenitor states in mouse skeletal muscle regeneration.Communications biology · 2021Article
- Appendage regeneration is context dependent at the cellular level.Open biology · 2021Article
Corrections and comments
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Authors and funding
17 authors at 9 institutions in 8 countries.
Funding
Abstract
While the capacity to regenerate tissues or limbs is limited in mammals, including humans, axolotls are able to regrow entire limbs and major organs after incurring a wound. The wound blastema has been extensively studied in limb regeneration. However, due to the inadequate characterization of ECM and cell subpopulations involved in the regeneration process, the discovery of the key drivers for human limb regeneration remains unknown. In this study, we applied large-scale single-cell RNA sequencing to classify cells throughout the adult axolotl limb regeneration process, uncovering a novel regeneration-specific mitochondria-related cluster supporting regeneration through energy providing and the ECM secretion (COL2+) cluster contributing to regeneration through cell-cell interactions signals. We also discovered the dedifferentiation and re-differentiation of the COL1+/COL2+ cellular subpopulation and exposed a COL2-mitochondria subcluster supporting the musculoskeletal system regeneration. On the basis of these findings, we reconstructed the dynamic single-cell transcriptome of adult axolotl limb regenerative process, and identified the novel regenerative mitochondria-related musculoskeletal populations, which yielded deeper insights into the crucial interactions between cell clusters within the regenerative microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.