ArticleBMC genomics2020
Label-free quantitative proteomic analysis of alfalfa in response to microRNA156 under high temperature.
Article in BMC genomics, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed, 13 citations in OpenAlex.
- Non-coding RNAs in plant stress responses: molecular insights and agricultural applications.Plant biotechnology journal · 2025Review
- Abiotic stress responses in forage crops and grasses: the role of secondary metabolites and biotechnological interventions.Frontiers in plant science · 2025Review
- MicroRNA: A Dynamic Player from Signalling to Abiotic Tolerance in Plants.International journal of molecular sciences · 2023Review
- TheInternational journal of molecular sciences · 2023Article
- Roles of microRNAs in abiotic stress response and characteristics regulation of plant.Frontiers in plant science · 2022Review
- MicroRNA-mediated bioengineering for climate-resilience in crops.Bioengineered · 2021Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAbiotic stress, including heat, is one of the major factors that affect alfalfa growth and forage yield. The small RNA, microRNA156 (miR156), regulates multiple traits in alfalfa during abiotic stress. The aim of this study was to explore the role of miR156 in regulating heat response in alfalfa at the protein level.
resultsIn this study, we compared an empty vector control and miR156 overexpressing (miR156OE) alfalfa plants after exposing them to heat stress (40 °C) for 24 h. We measured physiological parameters of control and miR156OE plants under heat stress, and collected leaf samples for protein analysis. A higher proline and antioxidant contents were detected in miR156OE plants than in controls under heat stress. Protein samples were analyzed by label-free quantification proteomics. Across all samples, a total of 1878 protein groups were detected. Under heat stress, 45 protein groups in the empty vector plants were significantly altered (P < 0.05; |log
conclusionsThese results suggest a positive role for miR156 in heat stress response in alfalfa. They reveal a miR156-regulated network of mechanisms at the protein level to modulate heat responses in alfalfa.
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Registered trials
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