ArticlePloS one2020
Single-cell analysis of transcriptome and DNA methylome in human oocyte maturation.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 2 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 2 syntheses or guidelines pooled it, 38 citations in OpenAlex.
- Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis.International journal of molecular sciences · 2023Pooled it
- Systematic review of mRNA expression in human oocytes: understanding the molecular mechanisms underlying oocyte competence.Journal of assisted reproduction and genetics · 2023Pooled it
- Novel multi-omic biomarkers to combat oocyte and ovarian aging.GeroScience · 2026Article
- Mapping ovarian cellular and molecular landscape across the lifespan of women: a scoping review.Human reproduction update · 2026Article
- Transcriptomic Analysis of Bovine Oocytes at GV and MII Stages and Dynamic Changes in Key Gene Expression Patterns.Biology · 2026Article
- Non-CG DNA methylation in animal genomes.Nature genetics · 2025Review
- Engineering strategies and challenges in building the follicular microenvironment for a bioprosthetic ovary.Best practice & research. Clinical obstetrics & gynaecology · 2025Review
- Discordant effects of maternal age on the human MII oocyte transcriptome.Molecular human reproduction · 2025Article
- DNA methylation mechanisms in the maturing and ageing oocyte.Epigenetics & chromatin · 2025Review
- Role of epigenetic regulation in diminished ovarian reserve.Journal of assisted reproduction and genetics · 2025Review
- Gestational diabetes mellitus causes genome hyper-methylation of oocyte via increased EZH2.Nature communications · 2025Article
- HIPSD&R-seq enables scalable genomic copy number and transcriptome profiling.Genome biology · 2024Article
- Regulation of Oocyte mRNA Metabolism: A Key Determinant of Oocyte Developmental Competence.Advances in anatomy, embryology, and cell biology · 2024Review
- Article
- Integrating imaging-based classification and transcriptomics for quality assessment of human oocytes according to their reproductive efficiency.Journal of assisted reproduction and genetics · 2023Article
- The developmental competence of human metaphase I oocytes with delayed maturation in vitro.Fertility and sterility · 2023Article
- Single-cell RNA-sequencing of retrieved human oocytes and eggs in clinical practice and for human ovarian cell atlasing.Molecular reproduction and development · 2022Review
- Unraveling the Puzzle: Oocyte Maturation Abnormalities (OMAS).Diagnostics (Basel, Switzerland) · 2022Review
- An Interplay between Epigenetics and Translation in Oocyte Maturation and Embryo Development: Assisted Reproduction Perspective.Biomedicines · 2022Review
- Epigenetics of pregnancy: looking beyond the DNA code.Journal of assisted reproduction and genetics · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Oocyte maturation is a coordinated process that is tightly linked to reproductive potential. A better understanding of gene regulation during human oocyte maturation will not only answer an important question in biology, but also facilitate the development of in vitro maturation technology as a fertility treatment. We generated single-cell transcriptome and used our previously published single-cell methylome data from human oocytes at different maturation stages to investigate how genes are regulated during oocyte maturation, focusing on the potential regulatory role of non-CpG methylation. DNMT3B, a gene encoding a key non-CpG methylation enzyme, is one of the 1,077 genes upregulated in mature oocytes, which may be at least partially responsible for the increased non-CpG methylation as oocytes mature. Non-CpG differentially methylated regions (DMRs) between mature and immature oocytes have multiple binding motifs for transcription factors, some of which bind with DNMT3B and may be important regulators of oocyte maturation through non-CpG methylation. Over 98% of non-CpG DMRs locate in transposable elements, and these DMRs are correlated with expression changes of the nearby genes. Taken together, this data indicates that global non-CpG hypermethylation during oocyte maturation may play an active role in gene expression regulation, potentially through the interaction with transcription factors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.