Evidence map›Paper›PMID 33159233›Full record

ArticleMolecular biology reports2020

Etv5 safeguards trophoblast stem cells differentiation from mouse EPSCs by regulating fibroblast growth factor receptor 2.

Kui Zhu, Yuan Liu, Chen Fan, Mengyao Zhang, Hongxia Cao, Xin He, Na Li, Dianfeng Chu, Fang Li, Min Zou and 5 more

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Article
  3. Single-nuclei RNA sequencing (snRNA-seq) uncovers trophoblast cell types and lineages in the mature bovine placenta.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 2 institutions in 1 country.

Kui ZhuCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Yuan LiuCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Chen FanCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Mengyao ZhangCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Hongxia CaoCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Xin HeCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Na LiCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Dianfeng ChuState Key Laboratory of Genetically Engineered Veterinary Vaccines, Yebio Bioengineering Co.Ltd of Qingdao, Qingdao, China.
Fang LiState Key Laboratory of Genetically Engineered Veterinary Vaccines, Yebio Bioengineering Co.Ltd of Qingdao, Qingdao, China.
Min ZouState Key Laboratory of Genetically Engineered Veterinary Vaccines, Yebio Bioengineering Co.Ltd of Qingdao, Qingdao, China.
Jinlian HuaCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Huayan WangCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Yan WangCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China.
Gencheng FanState Key Laboratory of Genetically Engineered Veterinary Vaccines, Yebio Bioengineering Co.Ltd of Qingdao, Qingdao, China. fangencheng@yebio.com.
Shiqiang ZhangCollege of Veterinary Medicine, Shaanxi Center of Stem Cells Engineering and Technology, Northwest A&F University, 712100, Yangling, China. shiqiangzhang@nwsuaf.edu.cn.ORCID http://orcid.org/0000-0002-0414-4365
North West Agriculture and Forestry University · CNYebio Bioengineering (China) · CN

Funding

National Natural Science Foundation of China 31571521, 31301218NWAFU Fundamental Research Funds for the Central Universities 2452019055State Key Laboratory of Genetically Engineered Veterinary Vaccines AGVSKL-ZY-201802, AGVSKL-ZD -202009
6 · The paper itself

Abstract

Previous studies have demonstrated that transcription factor Etv5 plays an important role in the segregation between epiblast and primitive endoderm at the second fate decision of early embryo. However, it remains elusive whether Etv5 functions in the segregation between inner cell mass and trophectoderm at the first cell fate decision. In this study, we firstly generated Etv5 knockout mouse embryonic stem cells (mESCs) by CRISPR/Cas9, then converted them into extended potential stem cells (EPSCs) by culturing the cells in small molecule cocktail medium LCDM (LIF, CHIR99021, (S)-(+)-dimethindene maleate, minocycline hydrochloride), and finally investigated their differentiation efficiency of trophoblast stem cells (TSCs). The results showed that Etv5 knockout significantly decreased the efficiency of TSCs (CDX2

Indexed as

AnimalsBenzamidesCell DifferentiationCells, CulturedCRISPR-Cas SystemsCulture MediaDimethindeneDiphenylamineDNA-Binding ProteinsEmbryonic DevelopmentGene Knockout TechniquesMAP Kinase Signaling SystemMiceMinocyclineMouse Embryonic Stem CellsReceptor, Fibroblast Growth Factor, Type 2BenzamidesCulture MediaDimethindeneDiphenylamineDNA-Binding ProteinsEtv5 protein, mouseFgfr2 protein, mouseMinocyclinemirdametinibReceptor, Fibroblast Growth Factor, Type 2Transcription FactorsEtv5Extended potential stem cellsFibroblast growth factor receptor 2Mouse embryonic stem cellsTrophoblast stem cells

Identifiers

PMID33159233
OpenAlexW3096049920

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.