ArticleJournal of neuroscience research2021
Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers.
Article in Journal of neuroscience research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- DKK1 Targeting in Corneal Schwann Cells Rescues Axonal Regeneration and Mechanosensory Function After Corneal Injury.Journal of neuroscience research · 2026Article
- Single cell RNA-seq characterization of non-fibrotic stromal wound repopulation in the rabbit.Experimental eye research · 2026Article
- Prediction of cell states and key transcription factors of the human cornea through integrated single-cell omics analyses.PNAS nexus · 2025Article
- Mind the GAPS: Glia associated with psychological stress.Journal of neuroendocrinology · 2025Review
- Single cell deciphering of pruritic keloids: the interaction between fibroblasts and Schwann cells through the Midkine signaling.Burns & trauma · 2025Article
- Mouse Corneal Immune Cell Heterogeneity Revealed by Single-Cell RNA Sequencing.Investigative ophthalmology & visual science · 2024Article
- Single cell analysis of short-term dry eye induced changes in cornea immune cell populations.Frontiers in medicine · 2024Article
- Review
- Single-cell transcriptomics of the ocular anterior segment: a comprehensive review.Eye (London, England) · 2023Review
- The miR-183/96/182 cluster is a checkpoint for resident immune cells and shapes the cellular landscape of the cornea.The ocular surface · 2023Article
- The Role of Sensory Innervation in Homeostatic and Injury-Induced Corneal Epithelial Renewal.International journal of molecular sciences · 2023Review
- Schwann Cells Are Key Regulators of Corneal Epithelial Renewal.Investigative ophthalmology & visual science · 2023Article
- Dry eye disease in mice activates adaptive corneal epithelial regeneration distinct from constitutive renewal in homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The transcriptional profile of keloidal Schwann cells.Experimental & molecular medicine · 2022Article
- Nimodipine Exerts Beneficial Effects on the Rat Oligodendrocyte Cell Line OLN-93.Brain sciences · 2022Article
- A single cell atlas of human cornea that defines its development, limbal progenitor cells and their interactions with the immune cells.The ocular surface · 2021Article
- Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers.Journal of neuroscience research · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
The cornea is the most innervated tissue in the human body. Myelinated axons upon inserting into the peripheral corneal stroma lose their myelin sheaths and continue into the central cornea wrapped by only nonmyelinating corneal Schwann cells (nm-cSCs). This anatomical organization is believed to be important for central vision. Here we employed single-cell RNA sequencing (scRNA-seq), microscopy, and transgenics to characterize these nm-cSCs of the central cornea. Using principal component analysis, uniform manifold approximation and projection, and unsupervised hierarchal cell clustering of scRNA-seq data derived from central corneal cells of male rabbits, we successfully identified several clusters representing different corneal cell types, including a unique cell cluster representing nm-cSCs. To confirm protein expression of cSC genes, we performed cross-species validation, employing corneal whole-mount immunostaining with confocal microscopy in mouse corneas. The expression of several representative proteins of nm-cSCs were validated. As the proteolipid protein 1 (PLP1) gene was also expressed in nm-cSCs, we explored the Plp1-eGFP transgenic reporter mouse line to visualize cSCs. Specific and efficient eGFP expression was observed in cSCs in adult mice of different ages. Of several putative cornea-specific SC genes identified, Dickkopf-related protein 1 was shown to be present in nm-cSCs. Taken together, our findings, for the first time, identify important insights and tools toward the study nm-cSCs in isolated tissue and adult animals. We expect that our results will advance the future study of nm-cSCs in applications of nerve repair, and provide a resource for the study of corneal sensory function.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.