Evidence map›Paper›PMID 33263572›Full record

ArticleActa crystallographica. Section F, Structural biology communications2020

ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: expression, purification and crystallization.

Lee S Izhaki-Tavor, Moshe Dessau

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lee S Izhaki-TavorAzrieli Faculty of Medicine in the Galilee, Bar-Ilan University, 1311502 Safed, Israel.ORCID 0000-0002-7520-7984
Moshe DessauAzrieli Faculty of Medicine in the Galilee, Bar-Ilan University, 1311502 Safed, Israel.

Funding

Israel Science Foundation 401/18
6 · The paper itself

Abstract

A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). Following expression, the protein was purified and crystallized using the vapor-diffusion method. The protein crystals were optimized at a 1:1 protein:reservoir solution ratio using PPGBA 2000. The optimized crystals diffracted to a d

Indexed as

Adenosine TriphosphateCrystallizationCrystallography, X-RayProtein DomainsProtozoan ProteinsRecombinant ProteinsRNA HelicasesTrypanosoma brucei bruceiAdenosine TriphosphateProtozoan ProteinsRecombinant ProteinsRNA HelicasesATP-dependent RNA helicasesDEAD-box proteinshelicase C domainprotozoan proteinsTrypanosoma bruceiZC3H41

Identifiers

PMID33263572
PMCPMC7716258

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.